{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["18(2)"],"submitter":["Blazevic T"],"pubmed_abstract":["We showed previously that capsaicin, an active compound of chili peppers, can inhibit platelet-derived growth factor-induced proliferation in primary rat vascular smooth muscle cells (VSMCs). The inhibition of BrdU incorporation by capsaicin in these cells was revoked by BCTC, which might be explained by a role of TRPV1 in VSMCs proliferation. To further pursue the hypothesis of a TRPV1-dependent effect of capsaicin, we investigated TRPV1 expression and function. Commercially available antibodies against two different TRPV1 epitopes (N-terminus and C-terminus) were rendered invalid in detecting TRPV1, as shown: i) in western blot experiments using control lysates of TRPV1-expressing (PC-12 and hTRPV1 transfected HEK293T) and TRPV1-downregulated (CRISPR/Cas gene edited A10) cells, and ii) b"],"journal":["PloS one"],"pagination":["e0281191"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9928102"],"repository":["biostudies-literature"],"pubmed_title":["Cultured rat aortic vascular smooth muscle cells do not express a functional TRPV1."],"pmcid":["PMC9928102"],"pubmed_authors":["Fischer MJM","Blazevic T","Ciotu CI","Dirsch VM","Gold-Binder M","Heiss EH"],"additional_accession":[]},"is_claimable":false,"name":"Cultured rat aortic vascular smooth muscle cells do not express a functional TRPV1.","description":"We showed previously that capsaicin, an active compound of chili peppers, can inhibit platelet-derived growth factor-induced proliferation in primary rat vascular smooth muscle cells (VSMCs). The inhibition of BrdU incorporation by capsaicin in these cells was revoked by BCTC, which might be explained by a role of TRPV1 in VSMCs proliferation. To further pursue the hypothesis of a TRPV1-dependent effect of capsaicin, we investigated TRPV1 expression and function. Commercially available antibodies against two different TRPV1 epitopes (N-terminus and C-terminus) were rendered invalid in detecting TRPV1, as shown: i) in western blot experiments using control lysates of TRPV1-expressing (PC-12 and hTRPV1 transfected HEK293T) and TRPV1-downregulated (CRISPR/Cas gene edited A10) cells, and ii) b","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023","modification":"2025-04-04T12:41:55.935Z","creation":"2025-04-04T12:41:55.935Z"},"accession":"S-EPMC9928102","cross_references":{"pubmed":["36787302"],"doi":["10.1371/journal.pone.0281191"]}}