{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Yu S"],"funding":["Ministry of Education","Korea University","National Research Foundation of Korea","BK21 FOUR program"],"pagination":["62-74"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9929172"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["39(1)"],"pubmed_abstract":["Plant pathogenic Pectobacterium species cause severe soft rot/blackleg diseases in many economically important crops worldwide. Pectobacterium utilizes plant cell wall degrading enzymes (PCWDEs) as the main virulence determinants for its pathogenicity. In this study, we screened a random mutant, M29 is a transposon insertion mutation in the metC gene encoding cystathionine β-lyase that catalyzes cystathionine to homocysteine at the penultimate step in methionine biosynthesis. M29 became a methionine auxotroph and resulted in growth defects in methionine-limited conditions. Impaired growth was restored with exogenous methionine or homocysteine rather than cystathionine. The mutant exhibited reduced soft rot symptoms in Chinese cabbages and potato tubers, maintaining activities of PCWDEs and"],"journal":["The plant pathology journal"],"pubmed_title":["Disruption of the metC Gene Affects Methionine Biosynthesis in Pectobacterium carotovorum subsp. carotovorum Pcc21 and Reduces Soft-Rot Disease."],"pmcid":["PMC9929172"],"funding_grant_id":["K2106871","2018R1D1A1A02085563","K2021521","4299991014324"],"pubmed_authors":["Chung EH","Yu S","Lee Y","Kang J"],"additional_accession":[]},"is_claimable":false,"name":"Disruption of the metC Gene Affects Methionine Biosynthesis in Pectobacterium carotovorum subsp. carotovorum Pcc21 and Reduces Soft-Rot Disease.","description":"Plant pathogenic Pectobacterium species cause severe soft rot/blackleg diseases in many economically important crops worldwide. Pectobacterium utilizes plant cell wall degrading enzymes (PCWDEs) as the main virulence determinants for its pathogenicity. In this study, we screened a random mutant, M29 is a transposon insertion mutation in the metC gene encoding cystathionine β-lyase that catalyzes cystathionine to homocysteine at the penultimate step in methionine biosynthesis. M29 became a methionine auxotroph and resulted in growth defects in methionine-limited conditions. Impaired growth was restored with exogenous methionine or homocysteine rather than cystathionine. The mutant exhibited reduced soft rot symptoms in Chinese cabbages and potato tubers, maintaining activities of PCWDEs and","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Feb","modification":"2025-04-04T12:43:05.971Z","creation":"2025-04-04T12:43:05.971Z"},"accession":"S-EPMC9929172","cross_references":{"pubmed":["36760050"],"doi":["10.5423/PPJ.OA.09.2022.0135"]}}