{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Srivastava P"],"funding":["National Health and Medical Research Council","NCI NIH HHS","National Institutes of Health","NIH HHS","Australian Research Council"],"pagination":["e2203614"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9929265"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["10(5)"],"pubmed_abstract":["Gastrulation is a stage in embryo development where three germ layers arise to dictate the human body plan. In vitro models of gastrulation have been demonstrated by treating pluripotent stem cells with soluble morphogens to trigger differentiation. However, in vivo gastrulation is a multistage process coordinated through feedback between soluble gradients and biophysical forces, with the multipotent epiblast transforming to the primitive streak followed by germ layer segregation. Here, the authors show how constraining pluripotent stem cells to hydrogel islands triggers morphogenesis that mirrors the stages preceding in vivo gastrulation, without the need for exogenous supplements. Within hours of initial seeding, cells display a contractile phenotype at the boundary, which leads to enhan"],"journal":["Advanced science (Weinheim, Baden-Wurttemberg, Germany)"],"pubmed_title":["Defined Microenvironments Trigger In Vitro Gastrulation in Human Pluripotent Stem Cells."],"pmcid":["PMC9929265"],"funding_grant_id":["R01CA251443","APP1185021","FT180100417","R01 CA251443"],"pubmed_authors":["Pimanda J","Ireland J","Chandrakanthan V","Nemec S","Yeola A","Romanazzo S","Kilian K","Molley TG","Jayathilaka PB","Kopecky C","Lin K","Pandzic E","Srivastava P"],"additional_accession":[]},"is_claimable":false,"name":"Defined Microenvironments Trigger In Vitro Gastrulation in Human Pluripotent Stem Cells.","description":"Gastrulation is a stage in embryo development where three germ layers arise to dictate the human body plan. In vitro models of gastrulation have been demonstrated by treating pluripotent stem cells with soluble morphogens to trigger differentiation. However, in vivo gastrulation is a multistage process coordinated through feedback between soluble gradients and biophysical forces, with the multipotent epiblast transforming to the primitive streak followed by germ layer segregation. Here, the authors show how constraining pluripotent stem cells to hydrogel islands triggers morphogenesis that mirrors the stages preceding in vivo gastrulation, without the need for exogenous supplements. Within hours of initial seeding, cells display a contractile phenotype at the boundary, which leads to enhan","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Feb","modification":"2026-07-14T20:23:05.521Z","creation":"2025-04-04T11:16:16.807Z"},"accession":"S-EPMC9929265","cross_references":{"pubmed":["36519269"],"doi":["10.1002/advs.202203614"]}}