{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wethekam LC"],"funding":["National Institutes of Health","University of Colorado Molecular Biology Training Program","NIH HHS","NIGMS NIH HHS"],"pagination":["e202202102"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9930134"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["222(3)"],"pubmed_abstract":["How cells regulate α- and β-tubulin to meet the demand for αβ-heterodimers and avoid consequences of monomer imbalance is not understood. We investigate the role of gene copy number and how shifting expression of α- or β-tubulin genes impacts tubulin proteostasis and microtubule function in Saccharomyces cerevisiae. We find that α-tubulin gene copy number is important for maintaining excess α-tubulin protein compared to β-tubulin protein. Excess α-tubulin prevents accumulation of super-stoichiometric β-tubulin, which leads to loss of microtubules, formation of non-microtubule assemblies of tubulin, and disrupts cell proliferation. In contrast, sub-stoichiometric β-tubulin or overexpression of α-tubulin has minor effects. We provide evidence that yeast cells equilibrate α-tubulin protein concentration when α-tubulin isotype expression is increased. We propose an asymmetric relationship between α- and β-tubulins, in which α-tubulins are maintained in excess to supply αβ-heterodimers and limit the accumulation of β-tubulin monomers."],"journal":["The Journal of cell biology"],"pubmed_title":["Tubulin isotype regulation maintains asymmetric requirement for α-tubulin over β-tubulin."],"pmcid":["PMC9930134"],"funding_grant_id":["T32 GM136444","R01 GM112893","Bolie Scholar Award","R35 GM 136253","R35 GM136253"],"pubmed_authors":["Wethekam LC","Moore JK"],"additional_accession":[]},"is_claimable":false,"name":"Tubulin isotype regulation maintains asymmetric requirement for α-tubulin over β-tubulin.","description":"How cells regulate α- and β-tubulin to meet the demand for αβ-heterodimers and avoid consequences of monomer imbalance is not understood. We investigate the role of gene copy number and how shifting expression of α- or β-tubulin genes impacts tubulin proteostasis and microtubule function in Saccharomyces cerevisiae. We find that α-tubulin gene copy number is important for maintaining excess α-tubulin protein compared to β-tubulin protein. Excess α-tubulin prevents accumulation of super-stoichiometric β-tubulin, which leads to loss of microtubules, formation of non-microtubule assemblies of tubulin, and disrupts cell proliferation. In contrast, sub-stoichiometric β-tubulin or overexpression of α-tubulin has minor effects. We provide evidence that yeast cells equilibrate α-tubulin protein concentration when α-tubulin isotype expression is increased. We propose an asymmetric relationship between α- and β-tubulins, in which α-tubulins are maintained in excess to supply αβ-heterodimers and limit the accumulation of β-tubulin monomers.","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Mar","modification":"2026-03-18T14:09:32.19Z","creation":"2025-04-04T22:55:17.112Z"},"accession":"S-EPMC9930134","cross_references":{"pubmed":["36719400"],"doi":["10.1083/jcb.202202102"]}}