biostudies-literatureMeng FChongqing Science and Technology Bureau, China553-562https://www.ebi.ac.uk/biostudies/studies/S-EPMC9930433biostudies-literatureUnknown27(4)Regulatory T cell (Treg) activity and differentiation in visceral adipose tissue (VAT) play an important role in inhibiting chronic inflammation and insulin resistance. Whether JAZF-1 and PPAR-γ mediate VAT Treg differentiation to promote the inhibition of chronic inflammation and insulin resistance remains unclear. Here, we investigated the roles of JAZF-1 and PPAR-γ in VAT Treg differentiation, inflammation and insulin resistance using a transgenic mouse model. First, we determined that the levels of glucose and insulin biochemical markers in the JAZF-1 transgenic general feeding or high-fat groups were lower than those in the wild-type general feeding or high-fat groups. Second, the levels of CD4⁺ , CD25⁺ , and FOXP3⁺ differentiation markers in the JAZF-Journal of cellular and molecular medicineRegulatory T cells differentiation in visceral adipose tissues contributes to insulin resistance by regulating JAZF-1/PPAR-γ pathway.PMC9930433cstc2019jcyj-msxmX0122Meng FHao PDu HfalseRegulatory T cells differentiation in visceral adipose tissues contributes to insulin resistance by regulating JAZF-1/PPAR-γ pathway.Regulatory T cell (Treg) activity and differentiation in visceral adipose tissue (VAT) play an important role in inhibiting chronic inflammation and insulin resistance. Whether JAZF-1 and PPAR-γ mediate VAT Treg differentiation to promote the inhibition of chronic inflammation and insulin resistance remains unclear. Here, we investigated the roles of JAZF-1 and PPAR-γ in VAT Treg differentiation, inflammation and insulin resistance using a transgenic mouse model. First, we determined that the levels of glucose and insulin biochemical markers in the JAZF-1 transgenic general feeding or high-fat groups were lower than those in the wild-type general feeding or high-fat groups. Second, the levels of CD4⁺ , CD25⁺ , and FOXP3⁺ differentiation markers in the JAZF-2023-01-01T00:00:00Z2023 Feb2025-04-18T13:38:04.303Z2025-04-06T23:24:54.739ZS-EPMC99304333673419810.1111/jcmm.17680