biostudies-literatureSun MChina National Tobacco CorporationNational Natural Science Foundation of China1101039https://www.ebi.ac.uk/biostudies/studies/S-EPMC9930646biostudies-literatureUnknown13Azoxystrobin, a quinone outside inhibitor fungicide, reduced tobacco target spot caused by <i>Rhizoctonia solani</i> by 62%, but also affected the composition and diversity of other microbes on the surface and interior of treated tobacco leaves. High-throughput sequencing showed that the dominant bacteria prior to azoxystrobin treatment were <i>Methylobacterium</i> on healthy leaves and <i>Pseudomonas</i> on diseased leaves, and the dominant fungi were <i>Thanatephorous</i> (teleomorph of <i>Rhizoctonia</i>) and <i>Symmetrospora</i> on healthy leaves and <i>Thanatephorous</i> on diseased leaves. Both bacterial and fungal diversity significantly increased 1 to 18 days post treatment (dpt) with azoxystrobin for healthy and diseased leaves. For bacteria on healthy leaves, the relative abundanFrontiers in plant scienceEffect of azoxystrobin on tobacco leaf microbial composition and diversity.PMC993064632160522 , 31960550110202001035(LS-04), 110202101048(LS-08), 110202101045(LS-05)Sun MLi JShi CCai LWang HChen XLiu TXiang LGoodwin PHWang LfalseEffect of azoxystrobin on tobacco leaf microbial composition and diversity.Azoxystrobin, a quinone outside inhibitor fungicide, reduced tobacco target spot caused by <i>Rhizoctonia solani</i> by 62%, but also affected the composition and diversity of other microbes on the surface and interior of treated tobacco leaves. High-throughput sequencing showed that the dominant bacteria prior to azoxystrobin treatment were <i>Methylobacterium</i> on healthy leaves and <i>Pseudomonas</i> on diseased leaves, and the dominant fungi were <i>Thanatephorous</i> (teleomorph of <i>Rhizoctonia</i>) and <i>Symmetrospora</i> on healthy leaves and <i>Thanatephorous</i> on diseased leaves. Both bacterial and fungal diversity significantly increased 1 to 18 days post treatment (dpt) with azoxystrobin for healthy and diseased leaves. For bacteria on healthy leaves, the relative abundan2022-01-01T00:00:00Z20222026-05-28T11:29:07.911Z2025-02-19T03:26:59.528ZS-EPMC99306463681648510.3389/fpls.2022.1101039