{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Verhulsel M"],"funding":["Fondation ARC pour la Recherche sur le Cancer","European Research Council","Agence Nationale de la Recherche","Institut National de la Santé et de la Recherche Médicale","H2020 Marie Skłodowska-Curie Actions"],"pagination":["365-377"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9930731"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["21(2)"],"pubmed_abstract":["Organoids are widely used as a model system to study gut pathophysiology; however, they fail to fully reproduce the complex, multi-component structure of the intestinal wall. We present here a new gut on chip model that allows the co-culture of primary epithelial and stromal cells. The device has the topography and dimensions of the mouse gut and is based on a 3D collagen I scaffold. The scaffold is coated with a thin layer of laminin to mimic the basement membrane. To maintain the scaffold structure while preserving its cytocompatibility, the collagen scaffold was rigidified by threose-based post-polymerization treatment. This treatment being cytocompatible enabled the incorporation of primary intestinal fibroblasts inside the scaffold, reproducing the gut stromal compartment. We observed"],"journal":["Lab on a chip"],"pubmed_title":["Developing an advanced gut on chip model enabling the study of epithelial cell/fibroblast interactions."],"pmcid":["PMC9930731"],"funding_grant_id":["ANR-10-EQPX-34","ANR-10-IDEX-0001-02 PSL","ANR-10-LABX-31","772487","321107","ANR Homeogut","666003"],"pubmed_authors":["Viovy JL","Simon A","Bernheim-Dennery M","Verhulsel M","Talini L","Geremie L","Descroix S","Gannavarapu VR","Krndija D","Ferraro D","Vignjevic DM"],"additional_accession":[]},"is_claimable":false,"name":"Developing an advanced gut on chip model enabling the study of epithelial cell/fibroblast interactions.","description":"Organoids are widely used as a model system to study gut pathophysiology; however, they fail to fully reproduce the complex, multi-component structure of the intestinal wall. We present here a new gut on chip model that allows the co-culture of primary epithelial and stromal cells. The device has the topography and dimensions of the mouse gut and is based on a 3D collagen I scaffold. The scaffold is coated with a thin layer of laminin to mimic the basement membrane. To maintain the scaffold structure while preserving its cytocompatibility, the collagen scaffold was rigidified by threose-based post-polymerization treatment. This treatment being cytocompatible enabled the incorporation of primary intestinal fibroblasts inside the scaffold, reproducing the gut stromal compartment. We observed","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Jan","modification":"2025-04-18T20:48:41.095Z","creation":"2024-11-05T22:24:30.116Z"},"accession":"S-EPMC9930731","cross_references":{"pubmed":["33306083"],"doi":["10.1039/d0lc00672f"]}}