biostudies-literatureVerhulsel MFondation ARC pour la Recherche sur le CancerEuropean Research CouncilAgence Nationale de la RechercheInstitut National de la Santé et de la Recherche MédicaleH2020 Marie Skłodowska-Curie Actions365-377https://www.ebi.ac.uk/biostudies/studies/S-EPMC9930731biostudies-literatureUnknown21(2)Organoids are widely used as a model system to study gut pathophysiology; however, they fail to fully reproduce the complex, multi-component structure of the intestinal wall. We present here a new gut on chip model that allows the co-culture of primary epithelial and stromal cells. The device has the topography and dimensions of the mouse gut and is based on a 3D collagen I scaffold. The scaffold is coated with a thin layer of laminin to mimic the basement membrane. To maintain the scaffold structure while preserving its cytocompatibility, the collagen scaffold was rigidified by threose-based post-polymerization treatment. This treatment being cytocompatible enabled the incorporation of primary intestinal fibroblasts inside the scaffold, reproducing the gut stromal compartment. We observedLab on a chipDeveloping an advanced gut on chip model enabling the study of epithelial cell/fibroblast interactions.PMC9930731ANR-10-EQPX-34ANR-10-IDEX-0001-02 PSLANR-10-LABX-31772487321107ANR Homeogut666003Viovy JLSimon ABernheim-Dennery MVerhulsel MTalini LGeremie LDescroix SGannavarapu VRKrndija DFerraro DVignjevic DMfalseDeveloping an advanced gut on chip model enabling the study of epithelial cell/fibroblast interactions.Organoids are widely used as a model system to study gut pathophysiology; however, they fail to fully reproduce the complex, multi-component structure of the intestinal wall. We present here a new gut on chip model that allows the co-culture of primary epithelial and stromal cells. The device has the topography and dimensions of the mouse gut and is based on a 3D collagen I scaffold. The scaffold is coated with a thin layer of laminin to mimic the basement membrane. To maintain the scaffold structure while preserving its cytocompatibility, the collagen scaffold was rigidified by threose-based post-polymerization treatment. This treatment being cytocompatible enabled the incorporation of primary intestinal fibroblasts inside the scaffold, reproducing the gut stromal compartment. We observed2021-01-01T00:00:00Z2021 Jan2025-04-18T20:48:41.095Z2024-11-05T22:24:30.116ZS-EPMC99307313330608310.1039/d0lc00672f