biostudies-literatureGhatak SNIDDK NIH HHSNINR NIH HHSNIGMS NIH HHS454-470https://www.ebi.ac.uk/biostudies/studies/S-EPMC9931555biostudies-literatureUnknown31(2)Fetal cutaneous wound closure and repair differ from that in adulthood. In this work, we identify an oxidant stress sensor protein, nonselenocysteine-containing phospholipid hydroperoxide glutathione peroxidase (NPGPx), that is abundantly expressed in normal fetal epidermis (and required for fetal wound closure), though not in adult epidermis, but is variably re-induced upon adult tissue wounding. NPGPx is a direct target of the miR-29 family. Following injury, abundance of miR-29 is lowered, permitting a prompt increase in NPGPx transcripts and protein expression in adult wound-edge tissue. NPGPx expression was required to mediate increased keratinocyte migration induced by miR-29 inhibition in vitro and in vivo. Increased NPGPx expression induced increased SOX2 expression and β-catenin nMolecular therapy : the journal of the American Society of Gene TherapyDriving adult tissue repair via re-engagement of a pathway required for fetal healing.PMC9931555R01 GM077185R56 DK076566R01 GM069589R01 NR013898K25 GM143572R01 GM108014R01 DK076566R01 DK114718Ghatak SWilgus TAWulff BCSen CKXuan YSharma APalakurti RGhosh NKhanna SRoy SEl Masry MSMaulik NThirunavukkarasu MPradeep SRYoder MCfalseDriving adult tissue repair via re-engagement of a pathway required for fetal healing.Fetal cutaneous wound closure and repair differ from that in adulthood. In this work, we identify an oxidant stress sensor protein, nonselenocysteine-containing phospholipid hydroperoxide glutathione peroxidase (NPGPx), that is abundantly expressed in normal fetal epidermis (and required for fetal wound closure), though not in adult epidermis, but is variably re-induced upon adult tissue wounding. NPGPx is a direct target of the miR-29 family. Following injury, abundance of miR-29 is lowered, permitting a prompt increase in NPGPx transcripts and protein expression in adult wound-edge tissue. NPGPx expression was required to mediate increased keratinocyte migration induced by miR-29 inhibition in vitro and in vivo. Increased NPGPx expression induced increased SOX2 expression and β-catenin n2023-01-01T00:00:00Z2023 Feb2025-05-29T21:29:39.935Z2025-05-29T21:29:39.935ZS-EPMC99315553611467310.1016/j.ymthe.2022.09.002