biostudies-literatureFanelli GItalian Ministry of Foreign Affairs and International Cooperation2692https://www.ebi.ac.uk/biostudies/studies/S-EPMC9931695biostudies-literatureUnknown13(1)The tripartite complex AcrAB-TolC is the major RND pump in Escherichia coli and other Enterobacteriaceae. It consists of the AcrB transporter, which is embedded in the inner membrane, the AcrA adapter located in the periplasm, and the channel protein TolC responsible for the transport of substrates towards the extracellular environment. Besides conferring resistance to many classes of antibiotics, AcrAB plays a role in the pathogenesis and virulence of several bacterial pathogens. Here we report that the AcrAB pump heavily affects the infection process of the LF82 strain, the prototype of Adherent-Invasive Escherichia coli (AIEC) which are highly abundant in the ileal mucosa of Chron disease patients. We found that the deletion of genes encoding AcrA and/or AcrB leads to decreased survivalScientific reportsAcrAB efflux pump impacts on the survival of adherent-invasive Escherichia coli strain LF82 inside macrophages.PMC9931695JP21GR04Colonna BProsseda GGrossi MFanelli GPasqua MfalseAcrAB efflux pump impacts on the survival of adherent-invasive Escherichia coli strain LF82 inside macrophages.The tripartite complex AcrAB-TolC is the major RND pump in Escherichia coli and other Enterobacteriaceae. It consists of the AcrB transporter, which is embedded in the inner membrane, the AcrA adapter located in the periplasm, and the channel protein TolC responsible for the transport of substrates towards the extracellular environment. Besides conferring resistance to many classes of antibiotics, AcrAB plays a role in the pathogenesis and virulence of several bacterial pathogens. Here we report that the AcrAB pump heavily affects the infection process of the LF82 strain, the prototype of Adherent-Invasive Escherichia coli (AIEC) which are highly abundant in the ileal mucosa of Chron disease patients. We found that the deletion of genes encoding AcrA and/or AcrB leads to decreased survival2023-01-01T00:00:00Z2023 Feb2025-04-20T03:04:19.484Z2025-04-20T03:04:19.484ZS-EPMC99316953679267210.1038/s41598-023-29817-0