biostudies-literatureUnknown120(3)Gryffroy LRhizogenic <i>Agrobacterium</i> strains comprise biotrophic pathogens that cause hairy root disease (HRD) on hydroponically grown <i>Solanaceae</i> and <i>Cucurbitaceae</i> crops, besides being widely explored agents for the creation of hairy root cultures for the sustainable production of plant-specialized metabolites. Hairy root formation is mediated through the expression of genes encoded on the T-DNA of the root-inducing (Ri) plasmid, of which several, including <i>root oncogenic locus B</i> (<i>rolB</i>), play a major role in hairy root development. Despite decades of research, the exact molecular function of the proteins encoded by the <i>rol</i> genes remains enigmatic. Here, by means of TurboID-mediated proximity labeling in tomato (<i>Solanum lycopersicum)</i> hairy roots, we idenProceedings of the National Academy of Sciences of the United States of Americae2210300120https://www.ebi.ac.uk/biostudies/studies/S-EPMC9934019biostudies-literatureRhizogenic <i>Agrobacterium</i> protein RolB interacts with the TOPLESS repressor proteins to reprogram plant immunity and development.PMC9934019De Coninck BDe Milde LJonckheere VVan Damme PGryffroy LCeulemans EManosalva Perez NJaramillo-Madrid ACVan Montagu MRodrigues SDVenegas-Molina JVandepoele KGoossens AfalseRhizogenic <i>Agrobacterium</i> protein RolB interacts with the TOPLESS repressor proteins to reprogram plant immunity and development.Rhizogenic <i>Agrobacterium</i> strains comprise biotrophic pathogens that cause hairy root disease (HRD) on hydroponically grown <i>Solanaceae</i> and <i>Cucurbitaceae</i> crops, besides being widely explored agents for the creation of hairy root cultures for the sustainable production of plant-specialized metabolites. Hairy root formation is mediated through the expression of genes encoded on the T-DNA of the root-inducing (Ri) plasmid, of which several, including <i>root oncogenic locus B</i> (<i>rolB</i>), play a major role in hairy root development. Despite decades of research, the exact molecular function of the proteins encoded by the <i>rol</i> genes remains enigmatic. Here, by means of TurboID-mediated proximity labeling in tomato (<i>Solanum lycopersicum)</i> hairy roots, we iden2023-01-01T00:00:00Z2023 Jan2025-04-26T15:15:39.723Z2025-04-06T14:48:38.568ZS-EPMC99340193663414210.1073/pnas.2210300120