{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Day JH"],"funding":["NIBIB NIH HHS","NIA NIH HHS","NIMH NIH HHS","NIH HHS"],"pubmed_abstract":["Expansion microscopy (ExM) enables nanoscale imaging using a standard confocal microscope through the physical, isotropic expansion of fixed immunolabeled specimens. ExM is widely employed to image proteins, nucleic acids, and lipid membranes in single cells at nanoscale resolution; however, current methods cannot be performed in multi-well cell culture plates which limits the number of samples that can be processed simultaneously. We developed High-throughput Expansion Microscopy (HiExM), a robust platform that enables expansion microscopy of cells cultured in a standard 96-well plate. Our method enables consistent ~4.2x expansion within individual wells, across multiple wells, and between plates processed in parallel. We also demonstrate that HiExM can be combined with high-throughput confocal imaging platforms greatly improve the ease and scalability of image acquisition. As an example, we analyzed the effects of doxorubicin, a known cardiotoxic agent, in human cardiomyocytes (CMs) based on Hoechst signal intensity. We show a dose dependent effect on nuclear chromatin that is not observed in unexpanded CMs, suggesting that HiExM improves the detection of cellular phenotypes in response to drug treatment. Our method broadens the application of ExM as a tool for scalable super-resolution imaging in biological research applications."],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2023.02.07.527509"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9934540"],"repository":["biostudies-literature"],"pubmed_title":["HiExM: high-throughput expansion microscopy enables scalable super-resolution imaging."],"pmcid":["PMC9934540"],"funding_grant_id":["R01 AG070831","S10 OD026839","R56 AG069192","R01 EB024261","RF1 MH124606","RF1 MH123403"],"pubmed_authors":["Schnieder KK","Boyden ES","Shin T","Boyer LA","Auld AL","Day JH","Della Santina CM","Maretich P"],"additional_accession":[]},"is_claimable":false,"name":"HiExM: high-throughput expansion microscopy enables scalable super-resolution imaging.","description":"Expansion microscopy (ExM) enables nanoscale imaging using a standard confocal microscope through the physical, isotropic expansion of fixed immunolabeled specimens. ExM is widely employed to image proteins, nucleic acids, and lipid membranes in single cells at nanoscale resolution; however, current methods cannot be performed in multi-well cell culture plates which limits the number of samples that can be processed simultaneously. We developed High-throughput Expansion Microscopy (HiExM), a robust platform that enables expansion microscopy of cells cultured in a standard 96-well plate. Our method enables consistent ~4.2x expansion within individual wells, across multiple wells, and between plates processed in parallel. We also demonstrate that HiExM can be combined with high-throughput confocal imaging platforms greatly improve the ease and scalability of image acquisition. As an example, we analyzed the effects of doxorubicin, a known cardiotoxic agent, in human cardiomyocytes (CMs) based on Hoechst signal intensity. We show a dose dependent effect on nuclear chromatin that is not observed in unexpanded CMs, suggesting that HiExM improves the detection of cellular phenotypes in response to drug treatment. Our method broadens the application of ExM as a tool for scalable super-resolution imaging in biological research applications.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Jan","modification":"2025-04-04T03:32:11.728Z","creation":"2025-02-19T01:21:06.271Z"},"accession":"S-EPMC9934540","cross_references":{"pubmed":["36798312"],"doi":["10.1101/2023.02.07.527509"]}}