biostudies-literatureUnknownStickel KCNCATS NIH HHSNEI NIH HHSNIDA NIH HHSNIDDK NIH HHSNCI NIH HHS<h4>Objective</h4>Spinophilin is an F-actin binding and protein phosphatase 1 (PP1) targeting protein that acts as a scaffold of PP1 to its substrates. Spinophilin knockout (Spino^-/-) mice have decreased fat mass, increased lean mass, and improved glucose tolerance, with no difference in feeding behaviors. While spinophilin is enriched in neurons, its roles in non-neuronal tissues, such as beta cells of the pancreatic islets, are unclear.<h4>Methods & results</h4>We have corroborated and expanded upon previous studies to determine that Spino^-/- mice have decreased weight gain and improved glucose tolerance in two different models of obesity. Using proteomics and immunoblotting-based approaches we identified multiple putative spinophilin interacting proteins isolated fbioRxiv : the preprint server for biology2023.02.07.527495https://www.ebi.ac.uk/biostudies/studies/S-EPMC9934546biostudies-literatureMechanisms of spinophilin-dependent pancreas dysregulation underlying diabesity.PMC9934546R33 DA041876R15 EY033968P30 CA082709R21 DA041876UL1 TR002529P30 DK097512Belecky-Adams TLBaucum AJDoud EHMosley ALStickel KCfalseMechanisms of spinophilin-dependent pancreas dysregulation underlying diabesity.<h4>Objective</h4>Spinophilin is an F-actin binding and protein phosphatase 1 (PP1) targeting protein that acts as a scaffold of PP1 to its substrates. Spinophilin knockout (Spino^-/-) mice have decreased fat mass, increased lean mass, and improved glucose tolerance, with no difference in feeding behaviors. While spinophilin is enriched in neurons, its roles in non-neuronal tissues, such as beta cells of the pancreatic islets, are unclear.<h4>Methods & results</h4>We have corroborated and expanded upon previous studies to determine that Spino^-/- mice have decreased weight gain and improved glucose tolerance in two different models of obesity. Using proteomics and immunoblotting-based approaches we identified multiple putative spinophilin interacting proteins isolated f2023-01-01T00:00:00Z2023 Feb2025-04-04T11:35:20.936Z2025-04-04T11:35:20.936ZS-EPMC99345463679836110.1101/2023.02.07.527495