biostudies-literatureUnknownMelotto GNIA NIH HHSNIH HHSThe cecidomyiid fly, soybean gall midge, Resseliella maxima Gagn√©, is a recently discovered insect that feeds on soybean plants in the Midwest US. Resseliella maxima larvae feed on soybean stems which may induce plant death and can cause considerable yield losses, making it an important agricultural pest. From three pools of 50 adults each, we used long-read nanopore sequencing to assemble a R. maxima reference genome. The final genome assembly is 206 Mb with 64.88X coverage, consisting of 1009 contigs with an N50 size of 714 kb. The assembly is high quality with a BUSCO score of 87.8%. Genome-wide GC level is 31.60% and DNA methylation was measured at 1.07%. The R. maxima genome is comprised of 21.73% repetitive DNA, which is in line with other cecidomyiids. PbioRxiv : the preprint server for biology2023.02.10.528044https://www.ebi.ac.uk/biostudies/studies/S-EPMC9934632biostudies-literatureThe Genome of the Soybean Gall Midge ( Resseliella maxima ).PMC9934632R21 AG071908T32 OD010993Faulk CWalls CBosley KMelotto GJacobson ELindsey ARIRamirez MJones MWKoch RLFlack NNelson SFrank LEKipp EJfalseThe Genome of the Soybean Gall Midge ( Resseliella maxima ).The cecidomyiid fly, soybean gall midge, Resseliella maxima Gagn√©, is a recently discovered insect that feeds on soybean plants in the Midwest US. Resseliella maxima larvae feed on soybean stems which may induce plant death and can cause considerable yield losses, making it an important agricultural pest. From three pools of 50 adults each, we used long-read nanopore sequencing to assemble a R. maxima reference genome. The final genome assembly is 206 Mb with 64.88X coverage, consisting of 1009 contigs with an N50 size of 714 kb. The assembly is high quality with a BUSCO score of 87.8%. Genome-wide GC level is 31.60% and DNA methylation was measured at 1.07%. The R. maxima genome is comprised of 21.73% repetitive DNA, which is in line with other cecidomyiids. P2023-01-01T00:00:00Z2023 Feb2026-04-08T14:36:50.969Z2025-02-18T23:45:21.172ZS-EPMC99346323679821010.1101/2023.02.10.528044