{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Camaglia F"],"funding":["CNRS-Weizmann","Agence Nationale de la Recherche","European Research Council"],"pagination":["e81622"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9934861"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12"],"pubmed_abstract":["One of the feats of adaptive immunity is its ability to recognize foreign pathogens while sparing the self. During maturation in the thymus, T cells are selected through the binding properties of their antigen-specific T-cell receptor (TCR), through the elimination of both weakly (positive selection) and strongly (negative selection) self-reactive receptors. However, the impact of thymic selection on the TCR repertoire is poorly understood. Here, we use transgenic Nur77-mice expressing a T-cell activation reporter to study the repertoires of thymic T cells at various stages of their development, including cells that do not pass selection. We combine high-throughput repertoire sequencing with statistical inference techniques to characterize the selection of the TCR in these distinct subsets"],"journal":["eLife"],"pubmed_title":["Quantifying changes in the T cell receptor repertoire during thymic development."],"pmcid":["PMC9934861"],"funding_grant_id":["80 prime","COG 724208","ANR-19-CE45-0018"],"pubmed_authors":["Ryvkin A","Chain B","Mora T","Reich-Zeliger S","Walczak AM","Camaglia F","Greenstein E","Friedman N"],"additional_accession":[]},"is_claimable":false,"name":"Quantifying changes in the T cell receptor repertoire during thymic development.","description":"One of the feats of adaptive immunity is its ability to recognize foreign pathogens while sparing the self. During maturation in the thymus, T cells are selected through the binding properties of their antigen-specific T-cell receptor (TCR), through the elimination of both weakly (positive selection) and strongly (negative selection) self-reactive receptors. However, the impact of thymic selection on the TCR repertoire is poorly understood. Here, we use transgenic Nur77-mice expressing a T-cell activation reporter to study the repertoires of thymic T cells at various stages of their development, including cells that do not pass selection. We combine high-throughput repertoire sequencing with statistical inference techniques to characterize the selection of the TCR in these distinct subsets","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jan","modification":"2025-04-20T03:03:39.529Z","creation":"2025-04-20T03:03:39.529Z"},"accession":"S-EPMC9934861","cross_references":{"pubmed":["36661220"],"doi":["10.7554/eLife.81622"]}}