{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["9(2)"],"submitter":["Lee KWC"],"pubmed_abstract":["<h4>Importance</h4>Molecular testing in non-small cell lung cancer (NSCLC) is commonly limited by inadequate tumor sample. Plasma cell-free DNA (cfDNA) genotyping as a complementary test is specific but only moderately sensitive. Genotyping of cfDNA in pleural and pericardial effusion (PE-cfDNA) can further optimize molecular diagnostic yield and reduce the need for repeated biopsies.<h4>Objective</h4>To prospectively validate droplet digital polymerase chain reaction (ddPCR) for detection of sensitizing EGFR variants and acquired Thr790Met variant (T790M) from PE-cfDNA in patients with NSCLC.<h4>Design, setting, and participants</h4>This prospective diagnostic validation study was conducted between September 6, 2016, and January 21, 2021 at 2 major Hong Kong cancer centers. Patients with "],"journal":["JAMA oncology"],"pagination":["261-265"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9936333"],"repository":["biostudies-literature"],"pubmed_title":["Testing for EGFR Variants in Pleural and Pericardial Effusion Cell-Free DNA in Patients With Non-Small Cell Lung Cancer."],"pmcid":["PMC9936333"],"pubmed_authors":["Lau YM","Mok TSK","Chan OSH","Chan VW","Kam KNP","Gai W","Lee KWC","Chan AKC","Lee CK","Fung SYH","Yeung RMW","Leung L","Cheung WF","Li MSC"],"additional_accession":[]},"is_claimable":false,"name":"Testing for EGFR Variants in Pleural and Pericardial Effusion Cell-Free DNA in Patients With Non-Small Cell Lung Cancer.","description":"<h4>Importance</h4>Molecular testing in non-small cell lung cancer (NSCLC) is commonly limited by inadequate tumor sample. Plasma cell-free DNA (cfDNA) genotyping as a complementary test is specific but only moderately sensitive. Genotyping of cfDNA in pleural and pericardial effusion (PE-cfDNA) can further optimize molecular diagnostic yield and reduce the need for repeated biopsies.<h4>Objective</h4>To prospectively validate droplet digital polymerase chain reaction (ddPCR) for detection of sensitizing EGFR variants and acquired Thr790Met variant (T790M) from PE-cfDNA in patients with NSCLC.<h4>Design, setting, and participants</h4>This prospective diagnostic validation study was conducted between September 6, 2016, and January 21, 2021 at 2 major Hong Kong cancer centers. Patients with ","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Feb","modification":"2025-04-19T14:32:31.902Z","creation":"2025-04-19T14:32:31.902Z"},"accession":"S-EPMC9936333","cross_references":{"pubmed":["36580285"],"doi":["10.1001/jamaoncol.2022.6109"]}}