{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Braxton E"],"funding":["Royal Society","Engineering and Physical Sciences Research Council"],"pagination":["21-31"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9936800"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["3(1)"],"pubmed_abstract":["For the detection of electrochemically produced hydroxyl radicals (HO<sup>·</sup>) from the oxidation of water on a boron-doped diamond (BDD) electrode, electron paramagnetic resonance spectroscopy (EPR) in combination with spin trap labels is a popular technique. Here, we show that quantification of the concentration of HO<sup>·</sup> from water oxidation via spin trap electrochemical (EC)-EPR is problematic. This is primarily due to the spin trap oxidizing at potentials less positive than water, resulting in the same spin trap-OH<sup>·</sup> adduct as formed from the solution reaction of OH<sup>·</sup> with the spin trap. We illustrate this through consideration of 5,5-dimethyl-1-pyrroline <i>N</i>-oxide (DMPO) as a spin trap for OH<sup>·</sup>. DMPO oxidation on a BDD electrode in an ac"],"journal":["ACS measurement science au"],"pubmed_title":["Electron Paramagnetic Resonance for the Detection of Electrochemically Generated Hydroxyl Radicals: Issues Associated with Electrochemical Oxidation of the Spin Trap."],"pmcid":["PMC9936800"],"funding_grant_id":["EP/V056778/1","EP/L015307/1","INF/PHD/180016","EP/L015315/1"],"pubmed_authors":["Fox DJ","Braxton E","Newton ME","Breeze BG","Tully JJ","Levey KJ","Macpherson JV"],"additional_accession":[]},"is_claimable":false,"name":"Electron Paramagnetic Resonance for the Detection of Electrochemically Generated Hydroxyl Radicals: Issues Associated with Electrochemical Oxidation of the Spin Trap.","description":"For the detection of electrochemically produced hydroxyl radicals (HO<sup>·</sup>) from the oxidation of water on a boron-doped diamond (BDD) electrode, electron paramagnetic resonance spectroscopy (EPR) in combination with spin trap labels is a popular technique. Here, we show that quantification of the concentration of HO<sup>·</sup> from water oxidation via spin trap electrochemical (EC)-EPR is problematic. This is primarily due to the spin trap oxidizing at potentials less positive than water, resulting in the same spin trap-OH<sup>·</sup> adduct as formed from the solution reaction of OH<sup>·</sup> with the spin trap. We illustrate this through consideration of 5,5-dimethyl-1-pyrroline <i>N</i>-oxide (DMPO) as a spin trap for OH<sup>·</sup>. DMPO oxidation on a BDD electrode in an ac","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Feb","modification":"2025-04-26T14:53:50.384Z","creation":"2025-04-06T14:46:34.357Z"},"accession":"S-EPMC9936800","cross_references":{"pubmed":["36817006"],"doi":["10.1021/acsmeasuresciau.2c00049"]}}