biostudies-literatureYamamoto TJapan Society for the Promotion of Sciencee0281746https://www.ebi.ac.uk/biostudies/studies/S-EPMC9937502biostudies-literatureUnknown18(2)The apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC)/caspase-1/interleukin(IL)-1β axis, also known as the inflammasome pathway, is indispensable for IL-1β activation in response to various pathogens or own damages. Previously, we developed an NLRP3-inflammasome using a cell-free system and identified ASC targeting drugs; thus, examination of ASC-related histopathology in various diseases could help to provide indications for these drugs. Here, we generated mice deficient only in ASC-protein (ASC-deficient (AD) mice) using CRISPR/Cas9 technology, studied which tissues were most affected, and obtained histopathological images of lipopolysaccharide (LPS)-induced endotoxemia. C57BL/6 wild-type (WT) and (AD) mice were injected intraperitoneally with a lethalPloS oneIntestinal edema induced by LPS-induced endotoxemia is associated with an inflammasome adaptor ASC.PMC993750220H03719, 22K1943820K07376Kaneko NKurata MMasumoto JYamamoto TfalseIntestinal edema induced by LPS-induced endotoxemia is associated with an inflammasome adaptor ASC.The apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC)/caspase-1/interleukin(IL)-1β axis, also known as the inflammasome pathway, is indispensable for IL-1β activation in response to various pathogens or own damages. Previously, we developed an NLRP3-inflammasome using a cell-free system and identified ASC targeting drugs; thus, examination of ASC-related histopathology in various diseases could help to provide indications for these drugs. Here, we generated mice deficient only in ASC-protein (ASC-deficient (AD) mice) using CRISPR/Cas9 technology, studied which tissues were most affected, and obtained histopathological images of lipopolysaccharide (LPS)-induced endotoxemia. C57BL/6 wild-type (WT) and (AD) mice were injected intraperitoneally with a lethal2023-01-01T00:00:00Z20232025-04-04T23:12:24.355Z2025-04-04T23:12:24.355ZS-EPMC99375023680032910.1371/journal.pone.0281746