biostudies-literatureGarofano KNCI NIH HHSNational Institutes of HealthNIH HHS2864https://www.ebi.ac.uk/biostudies/studies/S-EPMC9938282biostudies-literatureUnknown13(1)Platelets play a crucial role in cancer and thrombosis. However, the receptor-ligand repertoire mediating prostate cancer (PCa) cell-platelet interactions and ensuing consequences have not been fully elucidated. Microvilli emanating from the plasma membrane of PCa cell lines (RC77 T/E, MDA PCa 2b) directly contacted individual platelets and platelet aggregates. PCa cell-platelet interactions were associated with calcium mobilization in platelets, and translocation of P-selectin and integrin α_IIbβ₃ onto the platelet surface. PCa cell-platelet interactions reciprocally promoted PCa cell invasion and apoptotic resistance, and these events were insensitive to androgen receptor blockade by bicalutamide. PCa cells were exceedingly sensitive to activation by platelets in vitScientific reportsProstate cancer cell-platelet bidirectional signaling promotes calcium mobilization, invasion and apoptotic resistance via distinct receptor-ligand pairs.PMC9938282T32CA247756T32 CA247756U54MD010723R01 CA204806R01NS126090Garofano KLee NHSidahmed AGordon OSuwunnakorn SMaggirwar SBRashid KPopratiloff ABrantner CPerera MASmith MO'Brien TJHorvath ARhim JDiemert DKhan SfalseProstate cancer cell-platelet bidirectional signaling promotes calcium mobilization, invasion and apoptotic resistance via distinct receptor-ligand pairs.Platelets play a crucial role in cancer and thrombosis. However, the receptor-ligand repertoire mediating prostate cancer (PCa) cell-platelet interactions and ensuing consequences have not been fully elucidated. Microvilli emanating from the plasma membrane of PCa cell lines (RC77 T/E, MDA PCa 2b) directly contacted individual platelets and platelet aggregates. PCa cell-platelet interactions were associated with calcium mobilization in platelets, and translocation of P-selectin and integrin α_IIbβ₃ onto the platelet surface. PCa cell-platelet interactions reciprocally promoted PCa cell invasion and apoptotic resistance, and these events were insensitive to androgen receptor blockade by bicalutamide. PCa cells were exceedingly sensitive to activation by platelets in vit2023-01-01T00:00:00Z2023 Feb2026-07-14T20:07:00.428Z2025-04-19T02:45:05.74ZS-EPMC99382823680631510.1038/s41598-023-29450-x