biostudies-literatureUnknown101Rajput PNational Institute of Food and AgricultureMilk proteins serve as nutrition and affect neonate development and immunity through their bioactivity. Post-translational modifications of proteins affect their bioactivity. Glycosylation is the attachment of sugar moieties to proteins, with attachment of glycans to asparagine indicated as N-linked glycosylation. Our objective was to characterize N-linked glycosylated proteins in homogenate swine milk samples collected from sows (n = 5/6) during farrowing to represent colostrum and on days 3 and 14 post-farrowing to represent transitional and mature milk, respectively. Glycopeptides were isolated with lectin-based extraction and treated with Peptide N-glycosidase F (PNGase F) to identify N-linked glycosylation sites. Purified glycopeptides were analyzed by label-free liquid chromatographyJournal of animal scienceskac426https://www.ebi.ac.uk/biostudies/studies/S-EPMC9940737biostudies-literatureCharacterization of sow milk N-linked glycoproteome over the course of lactation.PMC9940737Minor RCAryal UKBhide KKrishnamurthy SRajput PCasey TMfalseCharacterization of sow milk N-linked glycoproteome over the course of lactation.Milk proteins serve as nutrition and affect neonate development and immunity through their bioactivity. Post-translational modifications of proteins affect their bioactivity. Glycosylation is the attachment of sugar moieties to proteins, with attachment of glycans to asparagine indicated as N-linked glycosylation. Our objective was to characterize N-linked glycosylated proteins in homogenate swine milk samples collected from sows (n = 5/6) during farrowing to represent colostrum and on days 3 and 14 post-farrowing to represent transitional and mature milk, respectively. Glycopeptides were isolated with lectin-based extraction and treated with Peptide N-glycosidase F (PNGase F) to identify N-linked glycosylation sites. Purified glycopeptides were analyzed by label-free liquid chromatography2023-01-01T00:00:00Z2023 Jan2026-05-18T03:15:27.822Z2025-04-04T19:01:33.66ZS-EPMC99407373658583710.1093/jas/skac426