{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Rudar M"],"funding":["Alabama Agricultural Experiment Station Agriculture Research Enhancement and Seed Funding","USDA National Institute of Food and Agriculture Hatch"],"pagination":["skad017"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9940738"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["101"],"pubmed_abstract":["Sulfur amino acid nutrition and metabolism are linked to animal disease. While validated methods for the determination of amino thiol levels in plasma or serum are available, there is a dearth of validated methods for their measurement in tissue. A robust and reproducible ultra-high performance liquid chromatography method has been validated for the simultaneous determination of concentrations of cysteine (Cys), cysteinylglycine (CysGly), homocysteine (Hcys), γ-glutamylcysteine (γ-GluCys), and glutathione (GSH) in pig tissue. Tissue was homogenized and deproteinized with trichloroacetic acid. Amino thiols in the acid-soluble fraction of the tissue homogenate were reduced with tris-(2-carboxyethyl)-phosphine hydrochloride and derivatized with 4-(aminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole "],"journal":["Journal of animal science"],"pubmed_title":["Technical note: simultaneous determination of amino thiols in pig tissue by ultra-high performance liquid chromatography with fluorescence detection."],"pmcid":["PMC9940738"],"funding_grant_id":["1025705"],"pubmed_authors":["Boersma M","Gachman A","Rudar M"],"additional_accession":[]},"is_claimable":false,"name":"Technical note: simultaneous determination of amino thiols in pig tissue by ultra-high performance liquid chromatography with fluorescence detection.","description":"Sulfur amino acid nutrition and metabolism are linked to animal disease. While validated methods for the determination of amino thiol levels in plasma or serum are available, there is a dearth of validated methods for their measurement in tissue. A robust and reproducible ultra-high performance liquid chromatography method has been validated for the simultaneous determination of concentrations of cysteine (Cys), cysteinylglycine (CysGly), homocysteine (Hcys), γ-glutamylcysteine (γ-GluCys), and glutathione (GSH) in pig tissue. Tissue was homogenized and deproteinized with trichloroacetic acid. Amino thiols in the acid-soluble fraction of the tissue homogenate were reduced with tris-(2-carboxyethyl)-phosphine hydrochloride and derivatized with 4-(aminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole ","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jan","modification":"2025-04-22T00:56:59.196Z","creation":"2025-04-05T19:43:45.89Z"},"accession":"S-EPMC9940738","cross_references":{"pubmed":["36630697"],"doi":["10.1093/jas/skad017"]}}