{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["6(1)"],"submitter":["Huayta J"],"pubmed_abstract":["In many organisms, dietary restriction (DR) leads to lifespan extension through the activation of cell protection and pro-longevity gene expression programs. In the nematode C. elegans, the DAF-16 transcription factor is a key aging regulator that governs the Insulin/IGF-1 signaling pathway and undergoes translocation from the cytoplasm to the nucleus of cells when animals are exposed to food limitation. However, how large is the influence of DR on DAF-16 activity, and its subsequent impact on lifespan has not been quantitatively determined. In this work, we assess the endogenous activity of DAF-16 under various DR regimes by coupling CRISPR/Cas9-enabled fluorescent tagging of DAF-16 with quantitative image analysis and machine learning. Our results indicate that DR regimes induce strong e"],"journal":["Communications biology"],"pagination":["203"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9941123"],"repository":["biostudies-literature"],"pubmed_title":["Endogenous DAF-16 spatiotemporal activity quantitatively predicts lifespan extension induced by dietary restriction."],"pmcid":["PMC9941123"],"pubmed_authors":["Crapster JP","San-Miguel A","Huayta J"],"additional_accession":[]},"is_claimable":false,"name":"Endogenous DAF-16 spatiotemporal activity quantitatively predicts lifespan extension induced by dietary restriction.","description":"In many organisms, dietary restriction (DR) leads to lifespan extension through the activation of cell protection and pro-longevity gene expression programs. In the nematode C. elegans, the DAF-16 transcription factor is a key aging regulator that governs the Insulin/IGF-1 signaling pathway and undergoes translocation from the cytoplasm to the nucleus of cells when animals are exposed to food limitation. However, how large is the influence of DR on DAF-16 activity, and its subsequent impact on lifespan has not been quantitatively determined. In this work, we assess the endogenous activity of DAF-16 under various DR regimes by coupling CRISPR/Cas9-enabled fluorescent tagging of DAF-16 with quantitative image analysis and machine learning. Our results indicate that DR regimes induce strong e","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Feb","modification":"2025-04-04T13:36:23.217Z","creation":"2025-02-18T22:55:47.034Z"},"accession":"S-EPMC9941123","cross_references":{"pubmed":["36807646"],"doi":["10.1038/s42003-023-04562-2"]}}