{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ali R"],"funding":["National Institutes of Health","NIH HHS","NIGMS NIH HHS"],"pagination":["e202202397"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9942271"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["28(70)"],"pubmed_abstract":["Phomoxanthone A is a naturally occurring molecule and a powerful anti-cancer agent, although its mechanism of action is unknown. To facilitate the determination of its biological target(s), we used affinity-based labelling using a phomoxanthone A probe. Labelled proteins were pulled down, subjected to chemoproteomics analysis using LC-MS/MS and ATP synthase was identified as a likely target. Mitochondrial ATP synthase was validated in cultured cells lysates and in live intact cells. Our studies show sixty percent inhibition of ATP synthase by 260 μM phomoxanthone A."],"journal":["Chemistry (Weinheim an der Bergstrasse, Germany)"],"pubmed_title":["Phomoxanthone A Targets ATP Synthase."],"pmcid":["PMC9942271"],"funding_grant_id":["R35GM124804","R35 GM124804","R35 GM118112"],"pubmed_authors":["Mitroka-Batsford S","Parelkar SS","Ali R","Coburn JM","Scarlata SF","Mattson AE","Thompson PR","Yerramilli S","Mistretta KS"],"additional_accession":[]},"is_claimable":false,"name":"Phomoxanthone A Targets ATP Synthase.","description":"Phomoxanthone A is a naturally occurring molecule and a powerful anti-cancer agent, although its mechanism of action is unknown. To facilitate the determination of its biological target(s), we used affinity-based labelling using a phomoxanthone A probe. Labelled proteins were pulled down, subjected to chemoproteomics analysis using LC-MS/MS and ATP synthase was identified as a likely target. Mitochondrial ATP synthase was validated in cultured cells lysates and in live intact cells. Our studies show sixty percent inhibition of ATP synthase by 260 μM phomoxanthone A.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Dec","modification":"2026-03-27T15:42:39.913Z","creation":"2025-04-04T11:20:31.233Z"},"accession":"S-EPMC9942271","cross_references":{"pubmed":["36082977"],"doi":["10.1002/chem.202202397"]}}