{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Itah Z"],"funding":["HHS | NIH | National Institute of Diabetes and Digestive and Kidney Diseases","NIDDK NIH HHS","Canada Research Chair in Molecular Oncology","Gouvernement du Canada | Canadian Institutes of Health Research","CIHR"],"pagination":["e2218373120"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9942916"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["120(4)"],"pubmed_abstract":["The HER2<sup>+</sup> subtype of human breast cancer is associated with the malignant transformation of luminal ductal cells of the mammary epithelium. The sequence analysis of tumor DNA identifies loss of function mutations and deletions of the <i>MAP2</i><i>K4</i> and <i>MAP2</i><i>K7</i> genes that encode direct activators of the JUN NH<sub>2</sub>-terminal kinase (JNK). We report that in vitro studies of human mammary epithelial cells with CRISPR-induced mutations in the MAPK and MAP2K components of the JNK pathway caused no change in growth in 2D culture, but these mutations promoted epithelial cell proliferation in 3D culture. Analysis of gene expression signatures in 3D culture demonstrated similar changes caused by HER2 activation and JNK pathway loss. The mechanism of signal transd"],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pubmed_title":["HER2-driven breast cancer suppression by the JNK signaling pathway."],"pmcid":["PMC9942916"],"funding_grant_id":["DK112698","DK107220","950·209844 X·216779","R01 DK112698","PJT-173401","CIHR FDN-148373","FDN-148373","CIHR PJT-173401","R01 DK107220"],"pubmed_authors":["Muller WJ","Davis RJ","Tournier C","Chaudhry S","Lee A","Aydemir O","Raju Ponny S","Cavanagh-Kyros J","Itah Z"],"additional_accession":[]},"is_claimable":false,"name":"HER2-driven breast cancer suppression by the JNK signaling pathway.","description":"The HER2<sup>+</sup> subtype of human breast cancer is associated with the malignant transformation of luminal ductal cells of the mammary epithelium. The sequence analysis of tumor DNA identifies loss of function mutations and deletions of the <i>MAP2</i><i>K4</i> and <i>MAP2</i><i>K7</i> genes that encode direct activators of the JUN NH<sub>2</sub>-terminal kinase (JNK). We report that in vitro studies of human mammary epithelial cells with CRISPR-induced mutations in the MAPK and MAP2K components of the JNK pathway caused no change in growth in 2D culture, but these mutations promoted epithelial cell proliferation in 3D culture. Analysis of gene expression signatures in 3D culture demonstrated similar changes caused by HER2 activation and JNK pathway loss. The mechanism of signal transd","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jan","modification":"2026-03-18T13:14:14.946Z","creation":"2025-08-18T09:53:03.494Z"},"accession":"S-EPMC9942916","cross_references":{"pubmed":["36656864"],"doi":["10.1073/pnas.2218373120"]}}