biostudies-literatureUnknown17(2)Xu N<h4>Background</h4>This study aims to evaluate the efficacy of the standard agglutination test (SAT), the Brucellacapt test and enzyme-linked immunosorbent assay (ELISA) in clinical specimens collected from patients with suspected brucellosis.<h4>Methods</h4>A prospective study was conducted from December 2020 to December 2021. Brucellosis was diagnosed on the basis of clinical evidence, and confirmed by isolation of Brucella or a four-fold rise in SAT titer. All samples were tested by the SAT, ELISA and the Brucellacapt test. Titers ≥1:100 were considered as SAT positive; ELISA was considered positive when an index greater than 11 was detected, while titers ≥1/160 indicated positivity on the Brucellacapt test. The specificity, sensitivity, and positive (PPVs) and negative predictive valuePLoS neglected tropical diseasese0011131https://www.ebi.ac.uk/biostudies/studies/S-EPMC9942959biostudies-literatureEvaluating the efficacy of serological testing of clinical specimens collected from patients with suspected brucellosis.PMC9942959Yang HLiu HQu CXu NYang LWang SSai LWen SWang GfalseEvaluating the efficacy of serological testing of clinical specimens collected from patients with suspected brucellosis.<h4>Background</h4>This study aims to evaluate the efficacy of the standard agglutination test (SAT), the Brucellacapt test and enzyme-linked immunosorbent assay (ELISA) in clinical specimens collected from patients with suspected brucellosis.<h4>Methods</h4>A prospective study was conducted from December 2020 to December 2021. Brucellosis was diagnosed on the basis of clinical evidence, and confirmed by isolation of Brucella or a four-fold rise in SAT titer. All samples were tested by the SAT, ELISA and the Brucellacapt test. Titers ≥1:100 were considered as SAT positive; ELISA was considered positive when an index greater than 11 was detected, while titers ≥1/160 indicated positivity on the Brucellacapt test. The specificity, sensitivity, and positive (PPVs) and negative predictive value2023-01-01T00:00:00Z2023 Feb2025-04-18T14:11:34.725Z2025-02-18T22:55:21.04ZS-EPMC99429593680239310.1371/journal.pntd.0011131