biostudies-literatureTakezawa YNICHD NIH HHSNINDS NIH HHS106013https://www.ebi.ac.uk/biostudies/studies/S-EPMC9945323biostudies-literatureUnknown178Neonatal hypoxia causes cytotoxic neuronal swelling by the entry of ions and water. Multiple water pathways have been implicated in neurons because these cells lack water channels, and their membrane has a low water permeability. NKCC1 and KCC2 are cation-chloride cotransporters (CCCs) involved in water movement in various cell types. However, the role of CCCs in water movement in neonatal neurons during hypoxia is unknown. We studied the effects of modulating CCCs pharmacologically on neuronal swelling in the neocortex (layer IV/V) of neonatal mice (post-natal day 8-13) during prolonged and brief hypoxia. We used acute brain slices from Clomeleon mice which express a ratiometric fluorophore sensitive to Cl^- and exposed them to oxygen-glucose deprivation (OGD) while imaging neurNeurobiology of diseaseRole of NKCC1 and KCC2 during hypoxia-induced neuronal swelling in the neonatal neocortex.PMC9945323P50 HD103556R01 NS115800Glykys JTakezawa YZimmerman MBBaule SMLangton RBaek SfalseRole of NKCC1 and KCC2 during hypoxia-induced neuronal swelling in the neonatal neocortex.Neonatal hypoxia causes cytotoxic neuronal swelling by the entry of ions and water. Multiple water pathways have been implicated in neurons because these cells lack water channels, and their membrane has a low water permeability. NKCC1 and KCC2 are cation-chloride cotransporters (CCCs) involved in water movement in various cell types. However, the role of CCCs in water movement in neonatal neurons during hypoxia is unknown. We studied the effects of modulating CCCs pharmacologically on neuronal swelling in the neocortex (layer IV/V) of neonatal mice (post-natal day 8-13) during prolonged and brief hypoxia. We used acute brain slices from Clomeleon mice which express a ratiometric fluorophore sensitive to Cl^- and exposed them to oxygen-glucose deprivation (OGD) while imaging neur2023-01-01T00:00:00Z2023 Mar2025-04-04T00:02:50.935Z2025-04-04T00:02:50.935ZS-EPMC99453233670692810.1016/j.nbd.2023.106013