{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Srednicka P"],"funding":["Prof. Wacław Dąbrowski Institute of Agricultural and Food Biotechnology – State Research Institute"],"pagination":["3026"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9945476"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(1)"],"pubmed_abstract":["Gut microbiota (GM) plays many key functions and helps maintain the host's health. Consequently, the development of GM cultivation under in vitro stimulating physiological conditions has gained extreme interest in different fields. In this study, we evaluated the impact of four culture media: Gut Microbiota Medium (GMM), Schaedler Broth (SM), Fermentation Medium (FM), and Carbohydrate Free Basal Medium (CFBM) on preserving the biodiversity and metabolic activity of human GM in batch in vitro cultures using PMA treatment coupled with 16S rDNA sequencing (PMA-seq) and LC-HR-MS/MS untargeted metabolomics supplemented with GC-MS SCFA profiling. Before the experiments, we determined the possibility of using the pooled faecal samples (MIX) from healthy donors (n = 15) as inoculum to reduce the n"],"journal":["Scientific reports"],"pubmed_title":["Effect of in vitro cultivation on human gut microbiota composition using 16S rDNA amplicon sequencing and metabolomics approach."],"pmcid":["PMC9945476"],"funding_grant_id":["project no. ZM-142-01"],"pubmed_authors":["Emanowicz P","Szczepanska M","Popowski D","Juszczuk-Kubiak E","Roszko ML","Kotyrba D","Kowalczyk M","Srednicka P","Wojcicki M"],"additional_accession":[]},"is_claimable":false,"name":"Effect of in vitro cultivation on human gut microbiota composition using 16S rDNA amplicon sequencing and metabolomics approach.","description":"Gut microbiota (GM) plays many key functions and helps maintain the host's health. Consequently, the development of GM cultivation under in vitro stimulating physiological conditions has gained extreme interest in different fields. In this study, we evaluated the impact of four culture media: Gut Microbiota Medium (GMM), Schaedler Broth (SM), Fermentation Medium (FM), and Carbohydrate Free Basal Medium (CFBM) on preserving the biodiversity and metabolic activity of human GM in batch in vitro cultures using PMA treatment coupled with 16S rDNA sequencing (PMA-seq) and LC-HR-MS/MS untargeted metabolomics supplemented with GC-MS SCFA profiling. Before the experiments, we determined the possibility of using the pooled faecal samples (MIX) from healthy donors (n = 15) as inoculum to reduce the n","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Feb","modification":"2026-04-07T13:22:49.587Z","creation":"2025-04-06T09:04:40.687Z"},"accession":"S-EPMC9945476","cross_references":{"pubmed":["36810418"],"doi":["10.1038/s41598-023-29637-2"]}}