biostudies-literatureUnknown131(2)Okamura T<h4>Background</h4>Microplastics (MPs) are small particles of plastic (≤5mm in diameter). In recent years, oral exposure to MPs in living organisms has been a cause of concern. Leaky gut syndrome (LGS), associated with a high-fat diet (HFD) in mice, can increase the entry of foreign substances into the body through the intestinal mucosa.<h4>Objectives</h4>We aimed to evaluate the pathophysiology of intestinal outcomes associated with consuming a high-fat diet and simultaneous intake of MPs, focusing on endocrine and metabolic systems.<h4>Methods</h4>C57BL6/J mice were fed a normal diet (ND) or HFD with or without polystyrene MP for 4 wk to investigate differences in glucose tolerance, intestinal permeability, gut microbiota, as well as metabolites in serum, feces, and liver.<h4>Results</h4Environmental health perspectives27006https://www.ebi.ac.uk/biostudies/studies/S-EPMC9945580biostudies-literatureOral Exposure to Polystyrene Microplastics of Mice on a Normal or High-Fat Diet and Intestinal and Metabolic Outcomes.PMC9945580Asano MYamazaki MSenmaru TNakanishi YFukui MMajima SSasano RHasegawa YTakano HOkamura THashimoto YNakanishi NHamaguchi MSeno HUshigome EfalseOral Exposure to Polystyrene Microplastics of Mice on a Normal or High-Fat Diet and Intestinal and Metabolic Outcomes.<h4>Background</h4>Microplastics (MPs) are small particles of plastic (≤5mm in diameter). In recent years, oral exposure to MPs in living organisms has been a cause of concern. Leaky gut syndrome (LGS), associated with a high-fat diet (HFD) in mice, can increase the entry of foreign substances into the body through the intestinal mucosa.<h4>Objectives</h4>We aimed to evaluate the pathophysiology of intestinal outcomes associated with consuming a high-fat diet and simultaneous intake of MPs, focusing on endocrine and metabolic systems.<h4>Methods</h4>C57BL6/J mice were fed a normal diet (ND) or HFD with or without polystyrene MP for 4 wk to investigate differences in glucose tolerance, intestinal permeability, gut microbiota, as well as metabolites in serum, feces, and liver.<h4>Results</h42023-01-01T00:00:00Z2023 Feb2026-07-14T22:23:57.193Z2025-04-06T11:43:59.912ZS-EPMC99455803682170810.1289/EHP11072