{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["10"],"submitter":["Liu Y"],"pubmed_abstract":["<h4>Background</h4>Rabies is a highly fatal disease. Once symptoms develop, death usually occurs within days. Survivors were occasionally reported in the literatures. Ante-mortem diagnosis remains a challenge in most rabies endemic countries. A novel, accurate diagnostic assay is highly desirable.<h4>Methods</h4>We used metagenomic next-generation sequencing (mNGS) to examine the cerebrospinal fluid (CSF) samples of a 49-year-old patient with rabies and validated the results by TaqMan PCR and RT-PCR/Sanger sequencing.<h4>Results</h4>Metagenomic next-generation sequencing identified sequence reads uniquely aligned to the rabies virus (RABV). PCR confirmed the presence of the partial RABV N gene in the CSF. Phylogenetic analysis showed that the RABV grouped as an Asian clade, which is the most broadly distributed clade in China.<h4>Conclusion</h4>Metagenomic next-generation sequencing may be a useful screening tool for the etiological diagnosis of rabies, especially in the absence of timely rabies laboratory testing or in patients with no exposure history."],"journal":["Frontiers in medicine"],"pagination":["982290"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9947348"],"repository":["biostudies-literature"],"pubmed_title":["Metagenomic next-generation sequencing for the etiological diagnosis of rabies virus in cerebrospinal fluid."],"pmcid":["PMC9947348"],"pubmed_authors":["Tian J","Liu Y","Li X","Willoughby RE","Feng Y","Weng X","Gao J","Wang Y","Mo X","Peng J"],"additional_accession":[]},"is_claimable":false,"name":"Metagenomic next-generation sequencing for the etiological diagnosis of rabies virus in cerebrospinal fluid.","description":"<h4>Background</h4>Rabies is a highly fatal disease. Once symptoms develop, death usually occurs within days. Survivors were occasionally reported in the literatures. Ante-mortem diagnosis remains a challenge in most rabies endemic countries. A novel, accurate diagnostic assay is highly desirable.<h4>Methods</h4>We used metagenomic next-generation sequencing (mNGS) to examine the cerebrospinal fluid (CSF) samples of a 49-year-old patient with rabies and validated the results by TaqMan PCR and RT-PCR/Sanger sequencing.<h4>Results</h4>Metagenomic next-generation sequencing identified sequence reads uniquely aligned to the rabies virus (RABV). PCR confirmed the presence of the partial RABV N gene in the CSF. Phylogenetic analysis showed that the RABV grouped as an Asian clade, which is the most broadly distributed clade in China.<h4>Conclusion</h4>Metagenomic next-generation sequencing may be a useful screening tool for the etiological diagnosis of rabies, especially in the absence of timely rabies laboratory testing or in patients with no exposure history.","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023","modification":"2026-06-25T03:24:52.352Z","creation":"2025-04-06T19:39:06.41Z"},"accession":"S-EPMC9947348","cross_references":{"pubmed":["36844226"],"doi":["10.3389/fmed.2023.982290"]}}