{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Park D"],"funding":["NEI NIH HHS","NICHD NIH HHS","OHSU ONPRC","NICHD","NCI NIH HHS","NIH","NIH HHS"],"pagination":["159-165"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9947459"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["3(2)"],"pubmed_abstract":["<h4>Objective</h4>To determine if the secretions collected from a conditionally reprogrammed primary endocervical cell culture are suitable surrogates for mucus studies.<h4>Design</h4>Experimental.<h4>Setting</h4>University research center.<h4>Animal(s)</h4>Female rhesus macaque (n = 2).<h4>Intervention(s)</h4>None.<h4>Main outcome measure(s)</h4>Quantitative proteomic analysis using tandem mass tag mass spectrometry liquid chromatography/tandem mass spectrometry.<h4>Result(s)</h4>We identified 3,047 proteins, common proteins present in both primary endocervical cell cultures and the mucus of rhesus macaques. We found a 71% overlap in the top 500 most prevalent proteins in the samples. Cell culture secretions contained many essential mucus proteins, including MUC5B, the primary mucin of th"],"journal":["F&S science"],"pubmed_title":["Mucus secretions from a conditionally reprogrammed primary endocervical cell culture."],"pmcid":["PMC9947459"],"funding_grant_id":["P30 CA069533","K12 HD000849","P30 EY010572","P51 OD011092","S10 OD012246"],"pubmed_authors":["Han L","Wilmarth PA","Reddy AP","Jensen JT","Park D"],"additional_accession":[]},"is_claimable":false,"name":"Mucus secretions from a conditionally reprogrammed primary endocervical cell culture.","description":"<h4>Objective</h4>To determine if the secretions collected from a conditionally reprogrammed primary endocervical cell culture are suitable surrogates for mucus studies.<h4>Design</h4>Experimental.<h4>Setting</h4>University research center.<h4>Animal(s)</h4>Female rhesus macaque (n = 2).<h4>Intervention(s)</h4>None.<h4>Main outcome measure(s)</h4>Quantitative proteomic analysis using tandem mass tag mass spectrometry liquid chromatography/tandem mass spectrometry.<h4>Result(s)</h4>We identified 3,047 proteins, common proteins present in both primary endocervical cell cultures and the mucus of rhesus macaques. We found a 71% overlap in the top 500 most prevalent proteins in the samples. Cell culture secretions contained many essential mucus proteins, including MUC5B, the primary mucin of th","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 May","modification":"2025-04-19T10:25:07.876Z","creation":"2025-02-19T02:37:48.027Z"},"accession":"S-EPMC9947459","cross_references":{"pubmed":["35560013"],"doi":["10.1016/j.xfss.2022.03.003"]}}