biostudies-literaturePark DNEI NIH HHSNICHD NIH HHSOHSU ONPRCNICHDNCI NIH HHSNIHNIH HHS159-165https://www.ebi.ac.uk/biostudies/studies/S-EPMC9947459biostudies-literatureUnknown3(2)<h4>Objective</h4>To determine if the secretions collected from a conditionally reprogrammed primary endocervical cell culture are suitable surrogates for mucus studies.<h4>Design</h4>Experimental.<h4>Setting</h4>University research center.<h4>Animal(s)</h4>Female rhesus macaque (n = 2).<h4>Intervention(s)</h4>None.<h4>Main outcome measure(s)</h4>Quantitative proteomic analysis using tandem mass tag mass spectrometry liquid chromatography/tandem mass spectrometry.<h4>Result(s)</h4>We identified 3,047 proteins, common proteins present in both primary endocervical cell cultures and the mucus of rhesus macaques. We found a 71% overlap in the top 500 most prevalent proteins in the samples. Cell culture secretions contained many essential mucus proteins, including MUC5B, the primary mucin of thF&S scienceMucus secretions from a conditionally reprogrammed primary endocervical cell culture.PMC9947459P30 CA069533K12 HD000849P30 EY010572P51 OD011092S10 OD012246Han LWilmarth PAReddy APJensen JTPark DfalseMucus secretions from a conditionally reprogrammed primary endocervical cell culture.<h4>Objective</h4>To determine if the secretions collected from a conditionally reprogrammed primary endocervical cell culture are suitable surrogates for mucus studies.<h4>Design</h4>Experimental.<h4>Setting</h4>University research center.<h4>Animal(s)</h4>Female rhesus macaque (n = 2).<h4>Intervention(s)</h4>None.<h4>Main outcome measure(s)</h4>Quantitative proteomic analysis using tandem mass tag mass spectrometry liquid chromatography/tandem mass spectrometry.<h4>Result(s)</h4>We identified 3,047 proteins, common proteins present in both primary endocervical cell cultures and the mucus of rhesus macaques. We found a 71% overlap in the top 500 most prevalent proteins in the samples. Cell culture secretions contained many essential mucus proteins, including MUC5B, the primary mucin of th2022-01-01T00:00:00Z2022 May2025-04-19T10:25:07.876Z2025-02-19T02:37:48.027ZS-EPMC99474593556001310.1016/j.xfss.2022.03.003