biostudies-literatureXu Lthe National Natural Science Foundation of China33https://www.ebi.ac.uk/biostudies/studies/S-EPMC9948446biostudies-literatureUnknown22(1)<h4>Background</h4>Existing methods for alkene epoxidation are based on lipase-catalysed perhydrolysis. However, the inactivation of the expensive lipase enzyme is problematic for enzymatic epoxidation at large scales due to the use of hydrogen peroxide and peracids at high concentrations in the reaction. The immobilisation of whole cells appears to be a promising approach to alleviate this problem.<h4>Results</h4>A green oxidation system containing hydrogen peroxide, Na₃C₆H₅O₇, an acyl donor, and glutaraldehyde (GA)-crosslinked cells of Rhizopus oryzae was developed for the epoxidation of alkenes. GA-crosslinked cells of Rhizopus oryzae were adopted as a biocatalyst into the epoxidation system. A variety of alkenes were oxidised with this systemMicrobial cell factoriesGlutaraldehyde-crosslinked Rhizopus oryzae whole cells show improved catalytic performance in alkene epoxidation.PMC9948446No. 21966007Xu LLiu YTang AQin YSong YfalseGlutaraldehyde-crosslinked Rhizopus oryzae whole cells show improved catalytic performance in alkene epoxidation.<h4>Background</h4>Existing methods for alkene epoxidation are based on lipase-catalysed perhydrolysis. However, the inactivation of the expensive lipase enzyme is problematic for enzymatic epoxidation at large scales due to the use of hydrogen peroxide and peracids at high concentrations in the reaction. The immobilisation of whole cells appears to be a promising approach to alleviate this problem.<h4>Results</h4>A green oxidation system containing hydrogen peroxide, Na₃C₆H₅O₇, an acyl donor, and glutaraldehyde (GA)-crosslinked cells of Rhizopus oryzae was developed for the epoxidation of alkenes. GA-crosslinked cells of Rhizopus oryzae were adopted as a biocatalyst into the epoxidation system. A variety of alkenes were oxidised with this system2023-01-01T00:00:00Z2023 Feb2026-03-18T13:16:19.106Z2025-05-29T21:12:00.882ZS-EPMC99484463681426810.1186/s12934-023-02026-0