biostudies-literatureUnknown13Takakura DAberrant glycosylation is a prominent feature of cancer, that can be used as targets to improve the existing cancer biomarkers, and help to assess metastasis risks, and therapeutic effects. We developed a targeted O-glycoproteomics method using serum specimens, and evaluated its utility in identifying advanced colorectal cancer (CRC) markers. To this end, we combined consecutive lectin affinity purification using <i>Maclura pomifera</i> lectin (MPL), jacalin, and <i>Sambucus nigra</i> lectin, which have affinities for the following O-glycans, that have received attention as cancer-related antigens, Tn (GalNAc-Ser/Thr), Sialyl Tn (Siaα2-6GalNAc-Ser/Thr), T (Galβ1-3GalNAc-Ser/Thr), Sialyl T (Siaα2-3Galβ1-GalNAc-Ser/Thr), and di-Sialyl T (Siaα2-3Galβ1-3[Siaα2-6] GalNAc-Ser/Thr), with a uniqueFrontiers in oncology1104936https://www.ebi.ac.uk/biostudies/studies/S-EPMC9948623biostudies-literatureTargeted O-glycoproteomics for the development of diagnostic markers for advanced colorectal cancer.PMC9948623Tokuhisa MIchikawa YKawasaki NTakakura DKobayashi NOhashi SfalseTargeted O-glycoproteomics for the development of diagnostic markers for advanced colorectal cancer.Aberrant glycosylation is a prominent feature of cancer, that can be used as targets to improve the existing cancer biomarkers, and help to assess metastasis risks, and therapeutic effects. We developed a targeted O-glycoproteomics method using serum specimens, and evaluated its utility in identifying advanced colorectal cancer (CRC) markers. To this end, we combined consecutive lectin affinity purification using <i>Maclura pomifera</i> lectin (MPL), jacalin, and <i>Sambucus nigra</i> lectin, which have affinities for the following O-glycans, that have received attention as cancer-related antigens, Tn (GalNAc-Ser/Thr), Sialyl Tn (Siaα2-6GalNAc-Ser/Thr), T (Galβ1-3GalNAc-Ser/Thr), Sialyl T (Siaα2-3Galβ1-GalNAc-Ser/Thr), and di-Sialyl T (Siaα2-3Galβ1-3[Siaα2-6] GalNAc-Ser/Thr), with a unique2023-01-01T00:00:00Z20232025-04-29T10:47:46.387Z2024-11-20T19:24:49.108ZS-EPMC99486233684568610.3389/fonc.2023.1104936