biostudies-literatureUnknownGeben LCNEI NIH HHSNICHD NIH HHSNIDDK NIH HHSNINDS NIH HHSNCI NIH HHSNIGMS NIH HHSA limiting factor in the regenerative capacity of the adult brain is the abundance and proliferative ability of neural stem cells (NSCs). Adult NSCs are derived from a subpopulation of embryonic NSCs that temporarily enter quiescence during mid-gestation and remain quiescent until postnatal reactivation. Here we present evidence that the mechanistic/mammalian target of rapamycin (mTOR) pathway regulates quiescence entry in embryonic NSCs of the developing forebrain. Throughout embryogenesis, two downstream effectors of mTOR, p-4EBP1/2 T37/46 and p-S6 S240/244, were mutually exclusive in NSCs, rarely occurring in the same cell. While 4EBP1/2 was phosphorylated in stem cells undergoing mitosis at the ventricular surface, S6 was phosphorylated in more differentiated cells migrating away from bioRxiv : the preprint server for biology2023.02.14.528513https://www.ebi.ac.uk/biostudies/studies/S-EPMC9948964biostudies-literatureDephosphorylation of 4EBP1/2 Induces Prenatal Neural Stem Cell Quiescence.PMC9948964T32 GM007628F31 NS120608F31 HD106890U24 DK059637P30 DK058404T32 HD007502P30 EY008126U54 CA217450P30 DK020593R01 DK106476P30 CA068485R01 NS118580R01 NS096238R01 CA226833Gallagher JEBrockman AAScheuing ALIrish JMEss KCChalkley MBLSimerly RBSweet SRIhrie RAGeben LCfalseDephosphorylation of 4EBP1/2 Induces Prenatal Neural Stem Cell Quiescence.A limiting factor in the regenerative capacity of the adult brain is the abundance and proliferative ability of neural stem cells (NSCs). Adult NSCs are derived from a subpopulation of embryonic NSCs that temporarily enter quiescence during mid-gestation and remain quiescent until postnatal reactivation. Here we present evidence that the mechanistic/mammalian target of rapamycin (mTOR) pathway regulates quiescence entry in embryonic NSCs of the developing forebrain. Throughout embryogenesis, two downstream effectors of mTOR, p-4EBP1/2 T37/46 and p-S6 S240/244, were mutually exclusive in NSCs, rarely occurring in the same cell. While 4EBP1/2 was phosphorylated in stem cells undergoing mitosis at the ventricular surface, S6 was phosphorylated in more differentiated cells migrating away from 2023-01-01T00:00:00Z2023 Feb2025-04-19T13:32:55.602Z2025-04-19T13:32:55.602ZS-EPMC99489643682476010.1101/2023.02.14.528513