biostudies-literatureUnknownIsei MONIA NIH HHSPhosphorylation of tau at sites associated with Alzheimer's disease (AD) likely plays a role in the disease progression. Mitochondrial impairment, correlating with increased presence of phosphorylated tau, has been identified as a contributing factor to neurodegenerative processes in AD. However, how tau phosphorylated at specific sites impacts mitochondrial function has not been fully defined. We examined how AD-relevant phosphomimetics of tau impact selected aspects of mitochondrial biology. To mimic phosphorylation at AD-associated sites, the Ser/Thr sites in wild-type GFP tagged-tau (T4) were converted to glutamic acid (E) to make pseudophosphorylated GFP tagged-Ser-396/404 (2EC) and GFP tagged-Thr-231/Ser-235 (2EM) constructs. These constructs were expressed in neuronal HT22 cells andbioRxiv : the preprint server for biology2023.02.19.529131https://www.ebi.ac.uk/biostudies/studies/S-EPMC9949115biostudies-literatureSite-specific phosphorylation of tau impacts mitochondrial biology and response to stressors.PMC9949115R01 AG067617Johnson GVRodwell-Bullock JIsei MOGirardi PANehrke KfalseSite-specific phosphorylation of tau impacts mitochondrial biology and response to stressors.Phosphorylation of tau at sites associated with Alzheimer's disease (AD) likely plays a role in the disease progression. Mitochondrial impairment, correlating with increased presence of phosphorylated tau, has been identified as a contributing factor to neurodegenerative processes in AD. However, how tau phosphorylated at specific sites impacts mitochondrial function has not been fully defined. We examined how AD-relevant phosphomimetics of tau impact selected aspects of mitochondrial biology. To mimic phosphorylation at AD-associated sites, the Ser/Thr sites in wild-type GFP tagged-tau (T4) were converted to glutamic acid (E) to make pseudophosphorylated GFP tagged-Ser-396/404 (2EC) and GFP tagged-Thr-231/Ser-235 (2EM) constructs. These constructs were expressed in neuronal HT22 cells and2023-01-01T00:00:00Z2023 Feb2025-04-18T12:50:11.094Z2025-02-19T04:54:55.188ZS-EPMC99491153682494010.1101/2023.02.19.529131