{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Robarts DR"],"funding":["NIA NIH HHS","NCRR NIH HHS","NIDDK NIH HHS","NIGMS NIH HHS","NIH HHS"],"pubmed_abstract":["<h4>Background & aims</h4>O-GlcNAcylation is a post-translational modification catalyzed by the enzyme O-GlcNAc transferase (OGT), which transfers a single N-acetylglucosamine sugar from UDP-GlcNAc to the protein on serine and threonine residues on proteins. Another enzyme, O-GlcNAcase (OGA), removes this modification. O-GlcNAcylation plays an important role in pathophysiology. Here, we report that O-GlcNAcylation is essential for hepatocyte differentiation, and chronic loss results in fibrosis and hepatocellular carcinoma.<h4>Methods</h4>Single-cell RNA-sequencing was used to investigate hepatocyte differentiation in hepatocyte-specific OGT-KO mice with increased hepatic O-GlcNAcylation and in OGA-KO mice with decreased O-GlcNAcylation in hepatocytes. HCC patient samples and the DEN-induc"],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2023.02.16.528884"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9949138"],"repository":["biostudies-literature"],"pubmed_title":["The Essential Role of O-GlcNAcylation in Hepatic Differentiation."],"pmcid":["PMC9949138"],"funding_grant_id":["P30 GM122731","P30 GM118247","R01 AG064227","P20 RR021940","P20 GM144269","S10 OD021743","R56 DK112768"],"pubmed_authors":["Slawson C","Hanover JA","Zachara NE","Paine-Cabrera D","Robarts DR","Venneman KK","Kotulkar M","Apte U"],"additional_accession":[]},"is_claimable":false,"name":"The Essential Role of O-GlcNAcylation in Hepatic Differentiation.","description":"<h4>Background & aims</h4>O-GlcNAcylation is a post-translational modification catalyzed by the enzyme O-GlcNAc transferase (OGT), which transfers a single N-acetylglucosamine sugar from UDP-GlcNAc to the protein on serine and threonine residues on proteins. Another enzyme, O-GlcNAcase (OGA), removes this modification. O-GlcNAcylation plays an important role in pathophysiology. Here, we report that O-GlcNAcylation is essential for hepatocyte differentiation, and chronic loss results in fibrosis and hepatocellular carcinoma.<h4>Methods</h4>Single-cell RNA-sequencing was used to investigate hepatocyte differentiation in hepatocyte-specific OGT-KO mice with increased hepatic O-GlcNAcylation and in OGA-KO mice with decreased O-GlcNAcylation in hepatocytes. HCC patient samples and the DEN-induc","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Feb","modification":"2026-04-08T12:26:53.299Z","creation":"2025-04-05T21:08:20.585Z"},"accession":"S-EPMC9949138","cross_references":{"pubmed":["36824917"],"doi":["10.1101/2023.02.16.528884"]}}