biostudies-literatureUnknownSchmied CNCI NIH HHSNIGMS NIH HHSImages document scientific discoveries and are prevalent in modern biomedical research. Microscopy imaging in particular is currently undergoing rapid technological advancements. However for scientists wishing to publish the obtained images and image analyses results, there are to date no unified guidelines. Consequently, microscopy images and image data in publications may be unclear or difficult to interpret. Here we present community-developed checklists for preparing light microscopy images and image analysis for publications. These checklists offer authors, readers, and publishers key recommendations for image formatting and annotation, color selection, data availability, and for reporting image analysis workflows. The goal of our guidelines is to increase the clarity and reproducibilArXivarXiv:2302.07005v2https://www.ebi.ac.uk/biostudies/studies/S-EPMC9949169biostudies-literatureCommunity-developed checklists for publishing images and image analyses.PMC9949169U01 CA200059P41 GM135019Grunwald DSchutz LAvilov SLacoste JConde-Sousa EChiritescu CBoehm UJambor HKHalidi NGaudreault NBakker GJEcker RSeitz AMontero Llopis PPayne-Dwyer ABrocher JParslow ACSelchow OBertocchi CHartley MSharma VPSpitaler MFernandez-Rodriguez JStrambio-De-Castillia CMiura KEliceiri KDevedec SLPlantard LSchroth-Diez BMartins GGTaatjes DLiu PNitschke RNorth AJug FChristopher JScott RTKoksoy AAHammer MCimini BASchmied CCarvalho MGelman LSrinivasan SBischof JHeld MGu TTischer CGuyader SLEbner MKapoor VNelson MSAli RMathur AfalseCommunity-developed checklists for publishing images and image analyses.Images document scientific discoveries and are prevalent in modern biomedical research. Microscopy imaging in particular is currently undergoing rapid technological advancements. However for scientists wishing to publish the obtained images and image analyses results, there are to date no unified guidelines. Consequently, microscopy images and image data in publications may be unclear or difficult to interpret. Here we present community-developed checklists for preparing light microscopy images and image analysis for publications. These checklists offer authors, readers, and publishers key recommendations for image formatting and annotation, color selection, data availability, and for reporting image analysis workflows. The goal of our guidelines is to increase the clarity and reproducibil2023-01-01T00:00:00Z2023 Sep2025-08-15T03:06:59.817Z2025-08-15T03:06:59.817ZS-EPMC994916936824427