{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Gatsios A"],"funding":["NCATS NIH HHS","Howard Hughes Medical Institute","National Institute of General Medical Sciences","NIGMS NIH HHS"],"pagination":["2626-2640"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9949963"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["85(11)"],"pubmed_abstract":["<i>Escherichia coli</i> isolates commonly inhabit the human microbiota, yet the majority of <i>E. coli</i>'s small-molecule repertoire remains uncharacterized. We previously employed erythromycin-induced translational stress to facilitate the characterization of autoinducer-3 (AI-3) and structurally related pyrazinones derived from \"abortive\" tRNA synthetase reactions in pathogenic, commensal, and probiotic <i>E. coli</i> isolates. In this study, we explored the \"missing\" tryptophan-derived pyrazinone reaction and characterized two other families of metabolites that were similarly upregulated under erythromycin stress. Strikingly, the abortive tryptophanyl-tRNA synthetase reaction leads to a tetracyclic indole alkaloid metabolite (<b>1</b>) rather than a pyrazinone. Furthermore, erythromyc"],"journal":["Journal of natural products"],"pubmed_title":["Cellular Stress-Induced Metabolites in <i>Escherichia coli</i>."],"pmcid":["PMC9949963"],"funding_grant_id":["1RM1GM141649-01","UL1 TR001863","RM1 GM141649"],"pubmed_authors":["Crawford JM","Kim CS","Flavell RA","York AG","Gatsios A"],"additional_accession":[]},"is_claimable":false,"name":"Cellular Stress-Induced Metabolites in <i>Escherichia coli</i>.","description":"<i>Escherichia coli</i> isolates commonly inhabit the human microbiota, yet the majority of <i>E. coli</i>'s small-molecule repertoire remains uncharacterized. We previously employed erythromycin-induced translational stress to facilitate the characterization of autoinducer-3 (AI-3) and structurally related pyrazinones derived from \"abortive\" tRNA synthetase reactions in pathogenic, commensal, and probiotic <i>E. coli</i> isolates. In this study, we explored the \"missing\" tryptophan-derived pyrazinone reaction and characterized two other families of metabolites that were similarly upregulated under erythromycin stress. Strikingly, the abortive tryptophanyl-tRNA synthetase reaction leads to a tetracyclic indole alkaloid metabolite (<b>1</b>) rather than a pyrazinone. Furthermore, erythromyc","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Nov","modification":"2026-06-06T21:12:14.611Z","creation":"2025-04-04T14:29:51.292Z"},"accession":"S-EPMC9949963","cross_references":{"pubmed":["36346625"],"doi":["10.1021/acs.jnatprod.2c00706"]}}