biostudies-literatureUnknown11(3)Xiao M<h4>Background</h4>Jian Pi Sheng Sui Gao (JPSSG), a Chinese traditional herbal paste, possesses certain efficacy in patients with cancer-related fatigue (CRF); however, its related mechanism remains unclear. Hence, network pharmacology analysis, followed by <i>in vivo</i> and <i>in vitro</i> experiments were conducted in this study with the aim to evaluate the effect of JPSSG on CRF and clarify its potential mechanism.<h4>Methods</h4>Network pharmacology analysis was performed. Subsequently, 12 mice were injected with CT26 cells to establish CRF mouse models and randomly divided into a model group (n=6) and JPSSG group (n=6); meanwhile, another 6 normal mice served as a control group. Then, 3.0 g/kg JPSSG was given to mice in JPSSG group for 15 days, while mice in the n control and model gAnnals of translational medicine156https://www.ebi.ac.uk/biostudies/studies/S-EPMC9951005biostudies-literatureJian Pi Sheng Sui Gao (JPSSG) alleviation of skeletal myoblast cell apoptosis, oxidative stress, and mitochondrial dysfunction to improve cancer-related fatigue in an AMPK-SIRT1- and HIF-1-dependent manner.PMC9951005Jiang JYang ZXiao MLi ZZhu YZhang JGuo WZhang CLin LShao CfalseJian Pi Sheng Sui Gao (JPSSG) alleviation of skeletal myoblast cell apoptosis, oxidative stress, and mitochondrial dysfunction to improve cancer-related fatigue in an AMPK-SIRT1- and HIF-1-dependent manner.<h4>Background</h4>Jian Pi Sheng Sui Gao (JPSSG), a Chinese traditional herbal paste, possesses certain efficacy in patients with cancer-related fatigue (CRF); however, its related mechanism remains unclear. Hence, network pharmacology analysis, followed by <i>in vivo</i> and <i>in vitro</i> experiments were conducted in this study with the aim to evaluate the effect of JPSSG on CRF and clarify its potential mechanism.<h4>Methods</h4>Network pharmacology analysis was performed. Subsequently, 12 mice were injected with CT26 cells to establish CRF mouse models and randomly divided into a model group (n=6) and JPSSG group (n=6); meanwhile, another 6 normal mice served as a control group. Then, 3.0 g/kg JPSSG was given to mice in JPSSG group for 15 days, while mice in the n control and model g2023-01-01T00:00:00Z2023 Feb2025-04-22T03:35:45.583Z2025-04-05T20:43:14.665ZS-EPMC99510053684600310.21037/atm-22-6611