{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Borner F"],"funding":["NCCIH NIH HHS","Deutsche Forschungsgemeinschaft","NHLBI NIH HHS","NIGMS NIH HHS"],"pagination":["e2205604"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9951388"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["10(6)"],"pubmed_abstract":["Specialized pro-resolving mediators (SPM), primarily produced in innate immune cells, exert crucial bioactions for resolving inflammation. Among various lipoxygenases (LOX), 15-LOX-1 is key for SPM biosynthesis, but cellular activation principles of 15-LOX-1 are unexplored. It was shown that 3-O-acetyl-11-keto-β-boswellic acid (AKBA) shifts 5-LOX regiospecificity from 5- to 12-lipoxygenation products. Here, it is demonstrated that AKBA additionally activates cellular 15-LOX-1 via an allosteric site accomplishing robust SPM formation in innate immune cells, particularly in M2 macrophages. Compared to ionophore, AKBA-induced LOX activation is Ca<sup>2+</sup> - and phosphorylation-independent, with modest induction of 5-LOX products. AKBA docks into a groove between the catalytic and regulato"],"journal":["Advanced science (Weinheim, Baden-Wurttemberg, Germany)"],"pubmed_title":["Allosteric Activation of 15-Lipoxygenase-1 by Boswellic Acid Induces the Lipid Mediator Class Switch to Promote Resolution of Inflammation."],"pmcid":["PMC9951388"],"funding_grant_id":["P50 AT002776","NIH HL107887","AHA 16GRNT31000010","NIH R15GM143724","SFB1278/1 Polytarget 316213987","SFB 1127/2 ChemBioSys 239748522","NIH P50AT002776","R15 GM143724","R01 HL107887"],"pubmed_authors":["Gilbert NC","Gerstmeier J","Gomez M","Jordan PM","Newcomer ME","Borner F","Rossi A","Pace S","Werz O"],"additional_accession":[]},"is_claimable":false,"name":"Allosteric Activation of 15-Lipoxygenase-1 by Boswellic Acid Induces the Lipid Mediator Class Switch to Promote Resolution of Inflammation.","description":"Specialized pro-resolving mediators (SPM), primarily produced in innate immune cells, exert crucial bioactions for resolving inflammation. Among various lipoxygenases (LOX), 15-LOX-1 is key for SPM biosynthesis, but cellular activation principles of 15-LOX-1 are unexplored. It was shown that 3-O-acetyl-11-keto-β-boswellic acid (AKBA) shifts 5-LOX regiospecificity from 5- to 12-lipoxygenation products. Here, it is demonstrated that AKBA additionally activates cellular 15-LOX-1 via an allosteric site accomplishing robust SPM formation in innate immune cells, particularly in M2 macrophages. Compared to ionophore, AKBA-induced LOX activation is Ca<sup>2+</sup> - and phosphorylation-independent, with modest induction of 5-LOX products. AKBA docks into a groove between the catalytic and regulato","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Feb","modification":"2026-05-29T05:28:03.832Z","creation":"2025-02-18T23:44:19.683Z"},"accession":"S-EPMC9951388","cross_references":{"pubmed":["36567268"],"doi":["10.1002/advs.202205604"]}}