biostudies-literatureUnknown12(2)Alam MBEach individual has a unique skin tone based on the types and quantities of melanin pigment, and oxidative stress is a key element in melanogenesis regulation. This research sought to understand the in vitro and in vivo antioxidant and depigmenting properties of betel leaves (<i>Piper betle</i> L.) extract (PBL) and the underlying mechanism. Ethyl acetate fractions of PBL (PBLA) demonstrated excellent phenolic content (342 ± 4.02 mgGAE/g) and strong DPPH, ABTS radicals, and nitric oxide (NO) scavenging activity with an IC₅₀ value of 41.52 ± 1.02 μg/mL, 45.60 ± 0.56 μg/mL, and 51.42 ± 1.25 μg/mL, respectively. Contrarily, ethanolic extract of PBL (PBLE) showed potent mushroom, mice, and human tyrosinase inhibition activity (IC₅₀ = 7.72 ± 0.98 μg/mL, 20.59 ± 0.83 μg/mL Antioxidants (Basel, Switzerland)374https://www.ebi.ac.uk/biostudies/studies/S-EPMC9952209biostudies-literatureAntioxidant Potential-Rich Betel Leaves (<i>Piper betle</i> L.) Exert Depigmenting Action by Triggering Autophagy and Downregulating MITF/Tyrosinase In Vitro and In Vivo.PMC9952209Park NHAlam MBSong BRLee SHfalseAntioxidant Potential-Rich Betel Leaves (<i>Piper betle</i> L.) Exert Depigmenting Action by Triggering Autophagy and Downregulating MITF/Tyrosinase In Vitro and In Vivo.Each individual has a unique skin tone based on the types and quantities of melanin pigment, and oxidative stress is a key element in melanogenesis regulation. This research sought to understand the in vitro and in vivo antioxidant and depigmenting properties of betel leaves (<i>Piper betle</i> L.) extract (PBL) and the underlying mechanism. Ethyl acetate fractions of PBL (PBLA) demonstrated excellent phenolic content (342 ± 4.02 mgGAE/g) and strong DPPH, ABTS radicals, and nitric oxide (NO) scavenging activity with an IC₅₀ value of 41.52 ± 1.02 μg/mL, 45.60 ± 0.56 μg/mL, and 51.42 ± 1.25 μg/mL, respectively. Contrarily, ethanolic extract of PBL (PBLE) showed potent mushroom, mice, and human tyrosinase inhibition activity (IC₅₀ = 7.72 ± 0.98 μg/mL, 20.59 ± 0.83 μg/mL 2023-01-01T00:00:00Z2023 Feb2025-04-05T13:20:31.534Z2025-02-18T23:57:40.579ZS-EPMC99522093682993310.3390/antiox12020374