biostudies-literatureCardozo GEspacio Interdisciplinario Universidad de la República UruguayPrograma de Alimentos y Salud Humana (PAyS) IDBPrograma de Desarrollo de Ciencias BásicasEspacio Interdisciplinario_2020 (to R.R.), Universidad de la República, UruguayComisión Sectorial de Investigación CientíficaPrograma de Alimentos y Salud Humana (PAyS) UruguaySistema Nacional de InvestigadoresComisión Sectorial de Investigación Científica Universidad de la República Uruguay408https://www.ebi.ac.uk/biostudies/studies/S-EPMC9952270biostudies-literatureUnknown12(2)Human peroxiredoxin 3 (<i>Hs</i>Prx3) is a thiol-based peroxidase responsible for the reduction of most hydrogen peroxide and peroxynitrite formed in mitochondria. Mitochondrial disfunction can lead to membrane lipoperoxidation, resulting in the formation of lipid-bound fatty acid hydroperoxides (_LFA-OOHs) which can be released to become free fatty acid hydroperoxides (_fFA-OOHs). Herein, we report that <i>Hs</i>Prx3 is oxidized and hyperoxidized by _fFA-OOHs including those derived from arachidonic acid and eicosapentaenoic acid peroxidation at position 15 with remarkably high rate constants of oxidation (>3.5 × 10⁷ M^-1s^-1) and hyperoxidation (~2 × 10⁷ M^-1s^-1). The endoperoxide-hydroperoxide PAntioxidants (Basel, Switzerland)Mitochondrial Peroxiredoxin 3 Is Rapidly Oxidized and Hyperoxidized by Fatty Acid Hydroperoxides.PMC9952270CSIC_2018IDB - R.O.U. (4950/OC-UR)GID2018_344950/OC-URCSIC i+d 2020 257Centros Interdisciplinarios 2020I+D 2020Cardozo GMastrogiovanni MTrujillo MRadi RViera NZeida AReyes AMfalseMitochondrial Peroxiredoxin 3 Is Rapidly Oxidized and Hyperoxidized by Fatty Acid Hydroperoxides.Human peroxiredoxin 3 (<i>Hs</i>Prx3) is a thiol-based peroxidase responsible for the reduction of most hydrogen peroxide and peroxynitrite formed in mitochondria. Mitochondrial disfunction can lead to membrane lipoperoxidation, resulting in the formation of lipid-bound fatty acid hydroperoxides (_LFA-OOHs) which can be released to become free fatty acid hydroperoxides (_fFA-OOHs). Herein, we report that <i>Hs</i>Prx3 is oxidized and hyperoxidized by _fFA-OOHs including those derived from arachidonic acid and eicosapentaenoic acid peroxidation at position 15 with remarkably high rate constants of oxidation (>3.5 × 10⁷ M^-1s^-1) and hyperoxidation (~2 × 10⁷ M^-1s^-1). The endoperoxide-hydroperoxide P2023-01-01T00:00:00Z2023 Feb2026-04-08T12:50:12.432Z2024-11-20T00:47:00.241ZS-EPMC99522703682996710.3390/antiox12020408