biostudies-literatureZani APFinancier of Studies and ProjectsNational Council for Scientific and Technological Development317https://www.ebi.ac.uk/biostudies/studies/S-EPMC9952489biostudies-literatureUnknown12(2)Cervical cancer is a health problem among women worldwide. Considering the limitations of prevention and antineoplastic chemotherapy against cervical cancer, research is needed to discover new, more effective, and safe antitumor agents. In the present study, we investigated the in vitro cytotoxicity of a new synthetic dibenzylideneacetone derived from 1,5-diaryl-3-oxo-1,4-pentadienyl (<b>A3K2A3</b>) against cervical cancer cells immortalized by HPV 16 (SiHa), and 18 (HeLa) by MTT assay. Furthermore, we performed spectrofluorimetry, flow cytometry, and Western blot analyzes to explore the inhibitory mechanism of <b>A3K2A3</b> in cervical cancer cells. <b>A3K2A3</b> showed cytotoxic activity against both cell lines. Mitochondrial depolarization and reduction in intracellular ATP levels were Antioxidants (Basel, Switzerland)Dibenzylideneacetone Induces Apoptosis in Cervical Cancer Cells through Ros-Mediated Mitochondrial Damage.PMC9952489421944/2018-8Garcia FPDin ZUNakamura CVZani APRodrigues-Filho EZani CPUeda-Nakamura Tde Oliveira Silva SfalseDibenzylideneacetone Induces Apoptosis in Cervical Cancer Cells through Ros-Mediated Mitochondrial Damage.Cervical cancer is a health problem among women worldwide. Considering the limitations of prevention and antineoplastic chemotherapy against cervical cancer, research is needed to discover new, more effective, and safe antitumor agents. In the present study, we investigated the in vitro cytotoxicity of a new synthetic dibenzylideneacetone derived from 1,5-diaryl-3-oxo-1,4-pentadienyl (<b>A3K2A3</b>) against cervical cancer cells immortalized by HPV 16 (SiHa), and 18 (HeLa) by MTT assay. Furthermore, we performed spectrofluorimetry, flow cytometry, and Western blot analyzes to explore the inhibitory mechanism of <b>A3K2A3</b> in cervical cancer cells. <b>A3K2A3</b> showed cytotoxic activity against both cell lines. Mitochondrial depolarization and reduction in intracellular ATP levels were 2023-01-01T00:00:00Z2023 Jan2025-04-05T07:24:30.351Z2025-04-05T07:24:30.351ZS-EPMC99524893682987610.3390/antiox12020317