{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Mariotti S"],"funding":["North Atlantic Treaty Organization","Istituto Superiore di Sanità","Italian Ministry of Defense"],"pagination":["610"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9953266"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["11(2)"],"pubmed_abstract":["The emergence of the new pathogen SARS-CoV-2 determined a rapid need for monoclonal antibodies (mAbs) to detect the virus in biological fluids as a rapid tool to identify infected individuals to be treated or quarantined. The majority of commercially available antigenic tests for SARS-CoV-2 rely on the detection of N antigen in biologic fluid using anti-N antibodies, and their capacity to specifically identify subjects infected by SARS-CoV-2 is questionable due to several structural analogies among the N proteins of different coronaviruses. In order to produce new specific antibodies, BALB/c mice were immunized three times at 20-day intervals with a recombinant spike (S) protein. The procedure used was highly efficient, and 40 different specific mAbs were isolated, purified and characteriz"],"journal":["Biomedicines"],"pubmed_title":["New Monoclonal Antibodies Specific for Different Epitopes of the Spike Protein of SARS-CoV-2 and Its Major Variants: Additional Tools for a More Specific COVID-19 Diagnosis."],"pmcid":["PMC9953266"],"funding_grant_id":["intramural funds","Emerging Security Challenges Division Science for Peace and Security Programme 2020: NO-COVID: New and validated tools for the diagnosis and follow-up of SARS-CoV-2 infected indi-viduals grant # G5817","Real-Biodefense, grant# a2018.92"],"pubmed_authors":["Michelini Z","Mariotti S","Marchi A","Bucci P","Capocefalo A","Gioacchini S","Negri D","Chiantore MV","Cara A","Di Bonito P","Iacobino A","D'Amelio R","Teloni R","Magurano F","Brouwer PJM","Grasso F","Borghi M","Baggieri M","Di Virgilio A","Sandini S","Nisini R","Acchioni C","Sgarbanti M"],"additional_accession":[]},"is_claimable":false,"name":"New Monoclonal Antibodies Specific for Different Epitopes of the Spike Protein of SARS-CoV-2 and Its Major Variants: Additional Tools for a More Specific COVID-19 Diagnosis.","description":"The emergence of the new pathogen SARS-CoV-2 determined a rapid need for monoclonal antibodies (mAbs) to detect the virus in biological fluids as a rapid tool to identify infected individuals to be treated or quarantined. The majority of commercially available antigenic tests for SARS-CoV-2 rely on the detection of N antigen in biologic fluid using anti-N antibodies, and their capacity to specifically identify subjects infected by SARS-CoV-2 is questionable due to several structural analogies among the N proteins of different coronaviruses. In order to produce new specific antibodies, BALB/c mice were immunized three times at 20-day intervals with a recombinant spike (S) protein. The procedure used was highly efficient, and 40 different specific mAbs were isolated, purified and characteriz","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Feb","modification":"2026-04-08T14:36:39.682Z","creation":"2024-11-20T08:07:05.729Z"},"accession":"S-EPMC9953266","cross_references":{"pubmed":["36831149"],"doi":["10.3390/biomedicines11020610"]}}