biostudies-literatureMariotti SNorth Atlantic Treaty OrganizationIstituto Superiore di SanitàItalian Ministry of Defense610https://www.ebi.ac.uk/biostudies/studies/S-EPMC9953266biostudies-literatureUnknown11(2)The emergence of the new pathogen SARS-CoV-2 determined a rapid need for monoclonal antibodies (mAbs) to detect the virus in biological fluids as a rapid tool to identify infected individuals to be treated or quarantined. The majority of commercially available antigenic tests for SARS-CoV-2 rely on the detection of N antigen in biologic fluid using anti-N antibodies, and their capacity to specifically identify subjects infected by SARS-CoV-2 is questionable due to several structural analogies among the N proteins of different coronaviruses. In order to produce new specific antibodies, BALB/c mice were immunized three times at 20-day intervals with a recombinant spike (S) protein. The procedure used was highly efficient, and 40 different specific mAbs were isolated, purified and characterizBiomedicinesNew Monoclonal Antibodies Specific for Different Epitopes of the Spike Protein of SARS-CoV-2 and Its Major Variants: Additional Tools for a More Specific COVID-19 Diagnosis.PMC9953266intramural fundsEmerging Security Challenges Division Science for Peace and Security Programme 2020: NO-COVID: New and validated tools for the diagnosis and follow-up of SARS-CoV-2 infected indi-viduals grant # G5817Real-Biodefense, grant# a2018.92Michelini ZMariotti SMarchi ABucci PCapocefalo AGioacchini SNegri DChiantore MVCara ADi Bonito PIacobino AD'Amelio RTeloni RMagurano FBrouwer PJMGrasso FBorghi MBaggieri MDi Virgilio ASandini SNisini RAcchioni CSgarbanti MfalseNew Monoclonal Antibodies Specific for Different Epitopes of the Spike Protein of SARS-CoV-2 and Its Major Variants: Additional Tools for a More Specific COVID-19 Diagnosis.The emergence of the new pathogen SARS-CoV-2 determined a rapid need for monoclonal antibodies (mAbs) to detect the virus in biological fluids as a rapid tool to identify infected individuals to be treated or quarantined. The majority of commercially available antigenic tests for SARS-CoV-2 rely on the detection of N antigen in biologic fluid using anti-N antibodies, and their capacity to specifically identify subjects infected by SARS-CoV-2 is questionable due to several structural analogies among the N proteins of different coronaviruses. In order to produce new specific antibodies, BALB/c mice were immunized three times at 20-day intervals with a recombinant spike (S) protein. The procedure used was highly efficient, and 40 different specific mAbs were isolated, purified and characteriz2023-01-01T00:00:00Z2023 Feb2026-04-08T14:36:39.682Z2024-11-20T08:07:05.729ZS-EPMC99532663683114910.3390/biomedicines11020610