{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Weber R"],"funding":["Swiss National Science Foundation","European Research Council"],"pagination":["638-650"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9957725"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["42(9)"],"pubmed_abstract":["Transcriptional and translational control are key determinants of gene expression, however, to what extent these two processes can be collectively coordinated is still poorly understood. Here, we use Nanopore long-read sequencing and cap analysis of gene expression (CAGE-seq) to document the landscape of 5' and 3' untranslated region (UTR) isoforms and transcription start sites of epidermal stem cells, wild-type keratinocytes and squamous cell carcinomas. Focusing on squamous cell carcinomas, we show that a small cohort of genes with alternative 5'UTR isoforms exhibit overall increased translational efficiencies and are enriched in ribosomal proteins and splicing factors. By combining polysome fractionations and CAGE-seq, we further characterize two of these UTR isoform genes with identica"],"journal":["Oncogene"],"pubmed_title":["Monitoring the 5'UTR landscape reveals isoform switches to drive translational efficiencies in cancer."],"pmcid":["PMC9957725"],"funding_grant_id":["176825","759006"],"pubmed_authors":["Bernasconi M","Spies D","Dure C","Ormiston M","Renz PF","Yamahachi H","Sendoel A","Taborsky D","Ghoshdastider U","Valdivia-Francia F","Forny M","Yigit M","Weber R"],"additional_accession":[]},"is_claimable":false,"name":"Monitoring the 5'UTR landscape reveals isoform switches to drive translational efficiencies in cancer.","description":"Transcriptional and translational control are key determinants of gene expression, however, to what extent these two processes can be collectively coordinated is still poorly understood. Here, we use Nanopore long-read sequencing and cap analysis of gene expression (CAGE-seq) to document the landscape of 5' and 3' untranslated region (UTR) isoforms and transcription start sites of epidermal stem cells, wild-type keratinocytes and squamous cell carcinomas. Focusing on squamous cell carcinomas, we show that a small cohort of genes with alternative 5'UTR isoforms exhibit overall increased translational efficiencies and are enriched in ribosomal proteins and splicing factors. By combining polysome fractionations and CAGE-seq, we further characterize two of these UTR isoform genes with identica","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Feb","modification":"2026-05-28T17:47:39.451Z","creation":"2024-11-08T20:27:12.441Z"},"accession":"S-EPMC9957725","cross_references":{"pubmed":["36550360"],"doi":["10.1038/s41388-022-02578-2"]}}