biostudies-literatureSun YXFundamental Research Funds for the Central UniversitiesOcean University of ChinaNational Natural Science Foundation of China106128https://www.ebi.ac.uk/biostudies/studies/S-EPMC9958412biostudies-literatureUnknown26(3)High-throughput determination of circadian rhythms in metabolic response and their divergent patterns in various microhabitats are crucial for understanding how organisms respond to environmental stresses. A mid-intertidal limpet <i>Cellana toreuma</i> was collected at various time points across both daytime and nighttime in winter during low tide for investigating the diurnal metabolomic responses to cold stress and elucidating the divergent metabolic responses to temperature variations across microhabitats. Temperatures of emergent rock microhabitats were lower than the tidal pool and even aggravated at night. A series of metabolomic responses exhibited coordinated diurnal changes in winter. Metabolic responses which were associated with cellular stress responses and energy metabolism ofiScienceMicrohabitat-specific diurnal metabolomic responses of the intertidal limpet <i>Cellana toreuma</i> to winter low temperature.PMC99584124202560441976142Dong YWSun YXHu LSfalseMicrohabitat-specific diurnal metabolomic responses of the intertidal limpet <i>Cellana toreuma</i> to winter low temperature.High-throughput determination of circadian rhythms in metabolic response and their divergent patterns in various microhabitats are crucial for understanding how organisms respond to environmental stresses. A mid-intertidal limpet <i>Cellana toreuma</i> was collected at various time points across both daytime and nighttime in winter during low tide for investigating the diurnal metabolomic responses to cold stress and elucidating the divergent metabolic responses to temperature variations across microhabitats. Temperatures of emergent rock microhabitats were lower than the tidal pool and even aggravated at night. A series of metabolomic responses exhibited coordinated diurnal changes in winter. Metabolic responses which were associated with cellular stress responses and energy metabolism of2023-01-01T00:00:00Z2023 Mar2025-08-27T03:06:44.473Z2025-04-05T23:09:56.481ZS-EPMC99584123685227310.1016/j.isci.2023.106128