{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Spruijtenburg B"],"funding":["São Paulo Research Foundation","Fundação de Amparo a Pesquisa do Estado de São Paulo-FAPESP","Canisius-Wilhelmina Ziekenhuis","Canisius-Wilhelmina Hospital"],"pagination":["207"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9958743"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["9(2)"],"pubmed_abstract":["<i>Candida tropicalis</i> is emerging as one of the most common <i>Candida</i> species causing opportunistic infections in Latin America. Outbreak events caused by <i>C. tropicalis</i> were reported, and antifungal resistant isolates are on the rise. In order to investigate population genomics and look into antifungal resistance, we applied a short tandem repeat (STR) genotyping scheme and antifungal susceptibility testing (AFST) to 230 clinical and environmental <i>C. tropicalis</i> isolates from Latin American countries. STR genotyping identified 164 genotypes, including 11 clusters comprised of three to seven isolates, indicating outbreak events. AFST identified one isolate as anidulafungin-resistant and harboring a <i>FKS1</i> S659P substitution. Moreover, we identified 24 clinical and environmental isolates with intermediate susceptibility or resistance to one or more azoles. <i>ERG11</i> sequencing revealed each of these isolates harboring a Y132F and/or Y257H/N substitution. All of these isolates, except one, were clustered together in two groups of closely related STR genotypes, with each group harboring distinct <i>ERG11</i> substitutions. The ancestral <i>C. tropicalis</i> strain of these isolates likely acquired the azole resistance-associated substitutions and subsequently spread across vast distances within Brazil. Altogether, this STR genotyping scheme for <i>C. tropicalis</i> proved to be useful for identifying unrecognized outbreak events and better understanding population genomics, including the spread of antifungal-resistant isolates."],"journal":["Journal of fungi (Basel, Switzerland)"],"pubmed_title":["Short Tandem Repeat Genotyping and Antifungal Susceptibility Testing of Latin American <i>Candida tropicalis</i> Isolates."],"pmcid":["PMC9958743"],"funding_grant_id":["CWZ_001421","001421","2017/02203-07"],"pubmed_authors":["Berrio I","Chaves GM","On Behalf Of The Latin American Group For Investigating Candida Tropicalis Resistance","Spruijtenburg B","Fernandez NB","de Groot T","Meijer EFJ","Baqueiro CCSZ","Colombo AL","de Almeida JN","Meis JF"],"additional_accession":[]},"is_claimable":false,"name":"Short Tandem Repeat Genotyping and Antifungal Susceptibility Testing of Latin American <i>Candida tropicalis</i> Isolates.","description":"<i>Candida tropicalis</i> is emerging as one of the most common <i>Candida</i> species causing opportunistic infections in Latin America. Outbreak events caused by <i>C. tropicalis</i> were reported, and antifungal resistant isolates are on the rise. In order to investigate population genomics and look into antifungal resistance, we applied a short tandem repeat (STR) genotyping scheme and antifungal susceptibility testing (AFST) to 230 clinical and environmental <i>C. tropicalis</i> isolates from Latin American countries. STR genotyping identified 164 genotypes, including 11 clusters comprised of three to seven isolates, indicating outbreak events. AFST identified one isolate as anidulafungin-resistant and harboring a <i>FKS1</i> S659P substitution. Moreover, we identified 24 clinical and environmental isolates with intermediate susceptibility or resistance to one or more azoles. <i>ERG11</i> sequencing revealed each of these isolates harboring a Y132F and/or Y257H/N substitution. All of these isolates, except one, were clustered together in two groups of closely related STR genotypes, with each group harboring distinct <i>ERG11</i> substitutions. The ancestral <i>C. tropicalis</i> strain of these isolates likely acquired the azole resistance-associated substitutions and subsequently spread across vast distances within Brazil. Altogether, this STR genotyping scheme for <i>C. tropicalis</i> proved to be useful for identifying unrecognized outbreak events and better understanding population genomics, including the spread of antifungal-resistant isolates.","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Feb","modification":"2025-08-27T03:10:39.374Z","creation":"2025-04-03T22:09:09.095Z"},"accession":"S-EPMC9958743","cross_references":{"pubmed":["36836321"],"doi":["10.3390/jof9020207"]}}