{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Mushtaq HMS"],"funding":["Deputyship for Research &amp;amp; Innovation, Ministry of Education in Saudi Arabia","Deputyship for Research & Innovation, Ministry of Education in Saudi Arabia"],"pagination":["192"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9959262"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(2)"],"pubmed_abstract":["DNA barcoding technology using short DNA sequences has emerged as an efficient and reliable tool for identifying, confirming, and resolving closely related taxa. This study used ITS2-rDNA and mtCOI DNA sequences to confirm the identity of eight <i>Oligonychus</i> species, representing 68 spider mite samples, collected mainly from Saudi Arabia (SA) and some from Mexico, Pakistan, USA, and Yemen. The intraspecific nucleotide divergences of the studied <i>Oligonychus</i> species ranged from 0% to 1.2% for ITS2 and 0% to 2.9% for COI. However, the interspecific nucleotide divergences were distinctly higher than the intraspecific ones and ranged from 3.7% to 51.1% for ITS2 and 3.2% to 18.1% for COI. Furthermore, molecular data correctly confirmed the species identity of 42 <i>Oligonychus</i> samples lacking males, including a previously claimed sample of <i>O. pratensis</i> from SA. High genetic variations were detected in two <i>Oligonychus</i> species: <i>O. afrasiaticus</i> (McGregor) (nine ITS2 and three COI haplotypes) and <i>O. tylus</i> Baker and Pritchard (four ITS2 and two COI haplotypes). In addition, ITS2- and COI-based phylogenetic trees confirmed the subdivision of the genus <i>Oligonychus</i>. In conclusion, integrative taxonomic approaches are vital to resolve the closely related <i>Oligonychus</i> species, identify the samples lacking male specimens, and assess phylogenetic relationships within and among species."],"journal":["Insects"],"pubmed_title":["Molecular-Based Taxonomic Inferences of Some Spider Mite Species of the Genus <i>Oligonychus</i> Berlese (Acari, Prostigmata, Tetranychidae)."],"pmcid":["PMC9959262"],"funding_grant_id":["IFKSURG-2-1157"],"pubmed_authors":["Alatawi FJ","Mushtaq HMS","Saleh AA","Kamran M"],"additional_accession":[]},"is_claimable":false,"name":"Molecular-Based Taxonomic Inferences of Some Spider Mite Species of the Genus <i>Oligonychus</i> Berlese (Acari, Prostigmata, Tetranychidae).","description":"DNA barcoding technology using short DNA sequences has emerged as an efficient and reliable tool for identifying, confirming, and resolving closely related taxa. This study used ITS2-rDNA and mtCOI DNA sequences to confirm the identity of eight <i>Oligonychus</i> species, representing 68 spider mite samples, collected mainly from Saudi Arabia (SA) and some from Mexico, Pakistan, USA, and Yemen. The intraspecific nucleotide divergences of the studied <i>Oligonychus</i> species ranged from 0% to 1.2% for ITS2 and 0% to 2.9% for COI. However, the interspecific nucleotide divergences were distinctly higher than the intraspecific ones and ranged from 3.7% to 51.1% for ITS2 and 3.2% to 18.1% for COI. Furthermore, molecular data correctly confirmed the species identity of 42 <i>Oligonychus</i> samples lacking males, including a previously claimed sample of <i>O. pratensis</i> from SA. High genetic variations were detected in two <i>Oligonychus</i> species: <i>O. afrasiaticus</i> (McGregor) (nine ITS2 and three COI haplotypes) and <i>O. tylus</i> Baker and Pritchard (four ITS2 and two COI haplotypes). In addition, ITS2- and COI-based phylogenetic trees confirmed the subdivision of the genus <i>Oligonychus</i>. In conclusion, integrative taxonomic approaches are vital to resolve the closely related <i>Oligonychus</i> species, identify the samples lacking male specimens, and assess phylogenetic relationships within and among species.","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Feb","modification":"2026-05-16T03:12:52.134Z","creation":"2025-02-18T23:44:27.763Z"},"accession":"S-EPMC9959262","cross_references":{"pubmed":["36835761"],"doi":["10.3390/insects14020192"]}}