biostudies-literatureXu QShuangbai Plan from Yantai Municipal CityTaishan Scholar Program from Shandong ProvinceNational Natural Science Foundation of China137https://www.ebi.ac.uk/biostudies/studies/S-EPMC9959855biostudies-literatureUnknown11(2)Benthic invertebrates produce secondary metabolites that serve as defenses against consumers and promote their fitness. To explore the chemical defense in marine benthic echinoderms, the chemical constituents of <i>Ophiopholis mirabilis</i> were investigated. A steroidal monoglycoside, asterosaponin P1, was isolated from <i>O. mirabilis</i> for the first-time using column chromatography. The chemical structure was characterized by spectroscopic and spectrometric methods. The embryotoxicity and teratogenicity of the isolated compound were assessed using the zebrafish embryo assay, a powerful vertebrate animal model system to study mechanisms of toxicity. When applied at high concentrations, asterosaponin P1 causes a significant increase in embryo mortality. A moderate LC₅₀ of astToxicsEmbryotoxicity and Teratogenicity of Steroidal Saponin Isolated from <i>Ophiopholis mirabilis</i>.PMC9959855tsqn20190403201802032270533Yang XLi XLiu YLi YMa BLin AYan ZXu QChen LHan SLi KZhang RfalseEmbryotoxicity and Teratogenicity of Steroidal Saponin Isolated from <i>Ophiopholis mirabilis</i>.Benthic invertebrates produce secondary metabolites that serve as defenses against consumers and promote their fitness. To explore the chemical defense in marine benthic echinoderms, the chemical constituents of <i>Ophiopholis mirabilis</i> were investigated. A steroidal monoglycoside, asterosaponin P1, was isolated from <i>O. mirabilis</i> for the first-time using column chromatography. The chemical structure was characterized by spectroscopic and spectrometric methods. The embryotoxicity and teratogenicity of the isolated compound were assessed using the zebrafish embryo assay, a powerful vertebrate animal model system to study mechanisms of toxicity. When applied at high concentrations, asterosaponin P1 causes a significant increase in embryo mortality. A moderate LC₅₀ of ast2023-01-01T00:00:00Z2023 Jan2025-04-22T02:28:12.93Z2025-04-05T20:20:52.021ZS-EPMC99598553685101210.3390/toxics11020137